Difference between revisions of "Part:BBa K5269020"
 
(2 intermediate revisions by one other user not shown)
Line 3: Line 3:
 
<partinfo>BBa_K5269020 short</partinfo>
 
<partinfo>BBa_K5269020 short</partinfo>
  
−
This sequence contains a tac promoter(BBa_K5269033), a BsmB I insert(BBa_K5269032), and an L3S2P21 terminator. This sequence was In-Fused to pBluescript II SK(-) In-Fusion forward primer 1 (BBa_K52690010) and pBluescript II SK(-) In-Fusion reverse primer 1 (BBa_K5269009). The two BsmB I insert(BBa_K5269032) were used for Golden Gate Assembly of the surface-presenting proteins (BBa_K5269021-BBa_K5269024).
+
This sequence contains a tac promoter, a BsmB I insert(BBa_K5269032), and a L3S2P21 terminator. This sequence was In-Fused to pBluescript II SK(-) In-Fusion forward primer 1 (BBa_K52690010) and pBluescript II SK(-) In-Fusion reverse primer 1 (BBa_K5269009). The two BsmB I insert(BBa_K5269032) were used for Golden Gate Assembly of the Surface displayed protein (BBa_K5269021-BBa_K5269024).
 +
 
 +
Here we show an image of the Golden Gate Assembly Site amplified by PCR and detected by electrophoresis. See the experiments page (https://2024.igem.wiki/tokyotech/experiments/) for details.
 +
 
 +
<html>
 +
<img src="https://static.igem.wiki/teams/5269/parts-registry/gga.webp" alt="SDS-PAGE Image" style="width:80%;>
 +
<br>
 +
</html>
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
Line 9: Line 16:
  
 
<!-- -->
 
<!-- -->
−
<span class='h3bb'>Sequence and Features</span>
+
<span class='h3bb'><br><br>Sequence and Features</span>
 
<partinfo>BBa_K5269020 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K5269020 SequenceAndFeatures</partinfo>
  

Latest revision as of 13:24, 2 October 2024


Golden Gate Assembly Site(tac promoter, BsmB I insert, L3S2P21)

This sequence contains a tac promoter, a BsmB I insert(BBa_K5269032), and a L3S2P21 terminator. This sequence was In-Fused to pBluescript II SK(-) In-Fusion forward primer 1 (BBa_K52690010) and pBluescript II SK(-) In-Fusion reverse primer 1 (BBa_K5269009). The two BsmB I insert(BBa_K5269032) were used for Golden Gate Assembly of the Surface displayed protein (BBa_K5269021-BBa_K5269024).

Here we show an image of the Golden Gate Assembly Site amplified by PCR and detected by electrophoresis. See the experiments page (https://2024.igem.wiki/tokyotech/experiments/) for details.

SDS-PAGE Image

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 84
    Illegal BamHI site found at 189
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]