Difference between revisions of "Part:BBa K5108000:Design"
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<h2>Design Notes</h2> | <h2>Design Notes</h2> | ||
| − | <p>BioBrick | + | <p>All BioBrick parts used for the are compatible with the RFC1000 Biobrick standard. Fusion of basic parts was performed following In-Fusion Assembly (Takara, France). </p> |
<h2>Source</h2> | <h2>Source</h2> | ||
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| − | = | + | <h2 style="color: blue;">References</h2> |
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| + | <li>UniProt. (s. d.-c). https://www.uniprot.org/uniprotkb/C3KDW3/entry</li> | ||
| + | <li>Trouillon J, Han K, Attrée I & Lory S (2022) The core and accessory Hfq interactomes across Pseudomonas aeruginosa lineages. Nat Commun 13: 1258 </li> | ||
| + | <li>Wu P, Wang Z, Zhu Q, Xie Z, Mei Y, Liang Y & Chen Z (2021) Stress preadaptation and overexpression of rpoS and hfq genes increase stress resistance of Pseudomonas fluorescens ATCC13525. Microbiological Research 250: 126804</li> | ||
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Latest revision as of 15:43, 29 September 2024
RNA binding protein Hfq from Pseudomonas fluorescens ATCC13525
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
All BioBrick parts used for the are compatible with the RFC1000 Biobrick standard. Fusion of basic parts was performed following In-Fusion Assembly (Takara, France).
Source
Synthetized from the genomic sequence of Pseudomonas fluorescens ATCC13525, codon optimized for Pseudomonas putida with Integrated DNA Technologies codon optimizer tool. This step permits to lower complexity and minimize secondary structures.