Difference between revisions of "Part:BBa K3022002:Experience"
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<I>Nanjing-China 2024 </I> | <I>Nanjing-China 2024 </I> | ||
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| − | We introduced PPK1 into Shewanella through a codon optimization method and found that it could work normally, and the introduction of PPK1 into Shewanella could also change the efficiency of electricity production by changing phosphorus metabolism | + | We introduced PPK1 into Shewanella through a codon optimization method and found that it could work normally, and the introduction of PPK1 into Shewanella could also change the efficiency of electricity production by changing phosphorus metabolism.We have also demonstrated that it is also capable of converting Pi to PolyP in Shewanella, and significantly affects the power production of Shewanella, as shown in the figure below. |
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| + | <img src="https://static.igem.wiki/teams/5034/engineering/fig12.png" style="width: 500px; height: auto;"> | ||
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| + | Figure 1: Electricity production capacity of <i>S. oneidensis.</i> after the introduction of PPK1 | ||
| + | We also used transmission electron microscopy (TEM) and scanning electron microscopy (SEM) to illustrate that if the protein is expressed in large quantities in Shewanella, it will lead to excessive intracellular phosphorus content, which will lead to changes in cell morphology. | ||
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| + | <img src="https://static.igem.wiki/teams/5034/engineering/fig6.png" style="width: 500px; height: auto;"> | ||
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| + | Figure 2: Scanning electron microscope of the <i>S. oneidensis.</i> morphology of WT and modified <i>S. oneidensis.</i> strains with Strong RBS BBa-B0034 | ||
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| + | <img src="https://static.igem.wiki/teams/5034/engineering/fig7.png" style="width: 500px; height: auto;"> | ||
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| + | Figure 3: Transmission electron microscopy of the <i>S. oneidensis.</i> morphology of WT and modified <i>S. oneidensis.</i> strains with Strong RBS(BBa-b0034) | ||
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| + | When the element was expressed in large quantities, the number of Shewanella decreased significantly, indicating that the mortality rate of Shewanella was significantly increased. | ||
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| + | <img src="https://static.igem.wiki/teams/5034/results/figure11.png" style="width: 500px; height: auto;"> | ||
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| + | Figure4：Growth curves of <i>S. oneidensis</i> at different rbs intensities | ||
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Latest revision as of 03:43, 1 October 2024
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The introduction of PPK1 into Shewanella could also change the efficiency of electricity production by changing phosphorus metabolism
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Nanjing-China 2024 |
We introduced PPK1 into Shewanella through a codon optimization method and found that it could work normally, and the introduction of PPK1 into Shewanella could also change the efficiency of electricity production by changing phosphorus metabolism.We have also demonstrated that it is also capable of converting Pi to PolyP in Shewanella, and significantly affects the power production of Shewanella, as shown in the figure below. 
When the element was expressed in large quantities, the number of Shewanella decreased significantly, indicating that the mortality rate of Shewanella was significantly increased. 
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