Difference between revisions of "Part:BBa K5398611"
 
(One intermediate revision by the same user not shown)
Line 5: Line 5:
 
<p>LicT<sup>CAT</sup>-ViViD fusion protein, also called LicV, is a transcriptional antitermination protein. It is composed of an N-terminal RNA-binding domain (coantiterminator (CAT)) derived from LicT and Vivid(VVD), a small LOV domain-containing protein that could form a dimeric structure quickly in blue light. Illumination could facilitate the dimerization of the fusion protein, consequently augmenting its affinity for the ribonucleic antiterminator (RAT) RNA sequence, thus preventing the formation of an RNA terminator stem–loop structure. When the light is removed, LicV doesn't bind to the RAT sequence, allowing the formation of the terminator hairpin structure which prevents the expression of genes downstream.</p>
 
<p>LicT<sup>CAT</sup>-ViViD fusion protein, also called LicV, is a transcriptional antitermination protein. It is composed of an N-terminal RNA-binding domain (coantiterminator (CAT)) derived from LicT and Vivid(VVD), a small LOV domain-containing protein that could form a dimeric structure quickly in blue light. Illumination could facilitate the dimerization of the fusion protein, consequently augmenting its affinity for the ribonucleic antiterminator (RAT) RNA sequence, thus preventing the formation of an RNA terminator stem–loop structure. When the light is removed, LicV doesn't bind to the RAT sequence, allowing the formation of the terminator hairpin structure which prevents the expression of genes downstream.</p>
  
−
<!-- Add more about the biology of this part here
+
<html lang="zh">
 +
<head>
 +
    <meta charset="UTF-8">
 +
    <meta name="viewport" content="width=device-width, initial-scale=1.0">
 +
    <style>
 +
        .module {
 +
            border: 1px solid #ccc; /* 边框 */
 +
            padding: 20px; /* 内边距 */
 +
            margin: 20px auto; /* 外边距，自动居中 */
 +
            width: 600px; /* 模块宽度 */
 +
            text-align: center; /* 内容居中 */
 +
            box-shadow: 0px 0px 10px rgba(0, 0, 0, 0.1); /* 阴影效果 */
 +
        }
 +
    </style>
 +
</head>
 +
<body>
 +
    <div class="module">
 +
        <img src="https://static.igem.wiki/teams/5398/improvement/20240930-130531.jpg" width="500" height="auto" alt="Improved EL222 system">
 +
        <p><b> Fig. 1 | Improved EL222 system with <i>LicV</i> and <i>RAT</i>. </b></p>
 +
    </div>
 +
</body>
 +
</html>
 +
 
 
===Usage and Biology===
 
===Usage and Biology===
 +
<p>The EL222-based blue light-inducible system allows precise control of gene expression by dimerizing and binding DNA upon blue light exposure. To reduce system leakage, a LicV fusion protein and RAT sequence were introduced, enhancing control by preventing premature transcription termination. This system is ideal for applications requiring tight regulation of gene expression in response to light, such as biosensors and metabolic engineering.</p>
  
 
<!-- -->
 
<!-- -->

Latest revision as of 13:22, 2 October 2024

LicTCAT-ViViD fusion protein


LicTCAT-ViViD fusion protein, also called LicV, is a transcriptional antitermination protein. It is composed of an N-terminal RNA-binding domain (coantiterminator (CAT)) derived from LicT and Vivid(VVD), a small LOV domain-containing protein that could form a dimeric structure quickly in blue light. Illumination could facilitate the dimerization of the fusion protein, consequently augmenting its affinity for the ribonucleic antiterminator (RAT) RNA sequence, thus preventing the formation of an RNA terminator stem–loop structure. When the light is removed, LicV doesn't bind to the RAT sequence, allowing the formation of the terminator hairpin structure which prevents the expression of genes downstream.

Improved EL222 system

Fig. 1 | Improved EL222 system with LicV and RAT.

Usage and Biology

The EL222-based blue light-inducible system allows precise control of gene expression by dimerizing and binding DNA upon blue light exposure. To reduce system leakage, a LicV fusion protein and RAT sequence were introduced, enhancing control by preventing premature transcription termination. This system is ideal for applications requiring tight regulation of gene expression in response to light, such as biosensors and metabolic engineering.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]