Difference between revisions of "Part:BBa K5291006"
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A gene from <i>Rhodopseudomonas palustris</i> CGA009 encoding a kind of carbonic anhydrase. | A gene from <i>Rhodopseudomonas palustris</i> CGA009 encoding a kind of carbonic anhydrase. | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
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| + | The gene <i>acaP</i> in <i>Rhodopseudomonas palustris</i> is able to encode a kind of α-CAs that can speed up the conversion between CO<sub>2</sub> and bicarbonate. By the method of <i>acaP</i> high copies, the rate of bicarbonate intake as well as the speed of bicarbonate- CO<sub>2</sub> conversion in <i>R. palustris</i> increase and the engineered bacteria is able to take advantage of carbon to produce cellulose.<br><br> | ||
| + | <html><img width = "550" src="https://static.igem.wiki/teams/5291/images/part-cr/acap-schema.png" /></html><br> | ||
| + | <b>Fig.1 The schema of <i>acaP</i>.</b><br><br> | ||
| + | <html><img width = "700" src="https://static.igem.wiki/teams/5291/images/part-cr/pbbr1mcs2-rca.png" /></html><br> | ||
| + | <b>Fig.2 The map of pBBR1MCS2-<i>acaP</i>.</b><br><br> | ||
| + | However, there is a great pity that we have not finished the verification of the function due to rush time.<br><br> | ||
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Latest revision as of 12:34, 2 October 2024
acaP
A gene from Rhodopseudomonas palustris CGA009 encoding a kind of carbonic anhydrase.
Usage and Biology
The gene acaP in Rhodopseudomonas palustris is able to encode a kind of α-CAs that can speed up the conversion between CO2 and bicarbonate. By the method of acaP high copies, the rate of bicarbonate intake as well as the speed of bicarbonate- CO2 conversion in R. palustris increase and the engineered bacteria is able to take advantage of carbon to produce cellulose.
Fig.1 The schema of acaP.
Fig.2 The map of pBBR1MCS2-acaP.
However, there is a great pity that we have not finished the verification of the function due to rush time.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 223
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 223
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 152
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 223
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 223
- 1000COMPATIBLE WITH RFC[1000]