Difference between revisions of "Part:BBa K5079016"
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<partinfo>BBa_K5079016 short</partinfo> | <partinfo>BBa_K5079016 short</partinfo> | ||
| − | + | This is the reverse primer for Part:BBa_K5079033. This is cloned in frame with the his tag, primers were used with the q5 polymerase master mix. The sequence is: GGTGCTCGAGTGCGGCCGCAGA TGAcaactggccacagtc. 3’ GC Content is 56%, 3' length is 18nt and 3' Tm is 65°C. | |
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Latest revision as of 09:16, 30 September 2024
K45_CP_N114 Switch primers into pET-28a Switch 8 (Rev)
This is the reverse primer for Part:BBa_K5079033. This is cloned in frame with the his tag, primers were used with the q5 polymerase master mix. The sequence is: GGTGCTCGAGTGCGGCCGCAGA TGAcaactggccacagtc. 3’ GC Content is 56%, 3' length is 18nt and 3' Tm is 65°C.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 12
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 5
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]