Difference between revisions of "Part:BBa K247004:Experience"
(→User Reviews) |
|||
(4 intermediate revisions by 3 users not shown) | |||
Line 18: | Line 18: | ||
<!-- End of the user review template --> | <!-- End of the user review template --> | ||
<!-- DON'T DELETE --><partinfo>BBa_K247004 EndReviews</partinfo> | <!-- DON'T DELETE --><partinfo>BBa_K247004 EndReviews</partinfo> | ||
+ | |||
This part properly acted as RFP reporter. | This part properly acted as RFP reporter. | ||
+ | |||
+ | Excitation peak: 584 nm, Emission peak: 607 nm | ||
+ | The Chiba iGEM team tested this part in several conditions. | ||
+ | |||
'''Experiment Condition''' | '''Experiment Condition''' | ||
*AHL concentration of medium : 100 nM | *AHL concentration of medium : 100 nM | ||
− | *Time when we observed mRFP fluorescence : 24 h | + | *Time when we observed mRFP fluorescence : 24 h |
− | *medium : 1(left) or 5 | + | *medium : 10 mL of LB-Amp,Cm medium containing 1(left) or 5(right) %(w/v) agar |
[[Image:Chiba_BBa_K247004.png|thumb|left|'''Fig. 1 RFP Fluorescent'''. RFP expression is induced by AHL (100 nM). Observed under UV irradiation.]] | [[Image:Chiba_BBa_K247004.png|thumb|left|'''Fig. 1 RFP Fluorescent'''. RFP expression is induced by AHL (100 nM). Observed under UV irradiation.]] |
Latest revision as of 04:09, 22 October 2009
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K247004
User Reviews
UNIQae9b4c3cc89fe216-partinfo-00000000-QINU UNIQae9b4c3cc89fe216-partinfo-00000001-QINU
This part properly acted as RFP reporter.
Excitation peak: 584 nm, Emission peak: 607 nm
The Chiba iGEM team tested this part in several conditions.
Experiment Condition
- AHL concentration of medium : 100 nM
- Time when we observed mRFP fluorescence : 24 h
- medium : 10 mL of LB-Amp,Cm medium containing 1(left) or 5(right) %(w/v) agar