Difference between revisions of "Part:BBa K5143027:Experience"
| (One intermediate revision by the same user not shown) | |||
| Line 1: | Line 1: | ||
__NOTOC__ | __NOTOC__ | ||
| − | |||
| − | |||
===Applications of BBa_K5143027=== | ===Applications of BBa_K5143027=== | ||
| + | <html lang="en"> | ||
| + | <p> | ||
| + | The Venus/Ruby plasmid has been used in our project to test the activation of the GAP promoter and the ADH1 promoter in the BY4741 <i> S. cerevisiae </i> strain by measuring the fluorescence. <br> | ||
| + | This plasmid has been digested by the XhoI restriction enzyme in order to release the Venus/Ruby fragment. Then, the Venus/Ruby fragment has been transformed in the BY4741 <i> S. cerevisiae </i> strain, and thanks to URA3_5' and URA3_3'homologous regions, this fragment has been inserted in the yeast genome by recombination.<br> | ||
| + | Moreover, the fluorescence of this new strain has been measured in order to test the activation of the GAP promoter and the ADH1 promoter in the construct. | ||
| + | </p> | ||
| + | </html lang="en"> | ||
===User Reviews=== | ===User Reviews=== | ||
Latest revision as of 09:53, 28 September 2024
Applications of BBa_K5143027
The Venus/Ruby plasmid has been used in our project to test the activation of the GAP promoter and the ADH1 promoter in the BY4741 S. cerevisiae strain by measuring the fluorescence.
This plasmid has been digested by the XhoI restriction enzyme in order to release the Venus/Ruby fragment. Then, the Venus/Ruby fragment has been transformed in the BY4741 S. cerevisiae strain, and thanks to URA3_5' and URA3_3'homologous regions, this fragment has been inserted in the yeast genome by recombination.
Moreover, the fluorescence of this new strain has been measured in order to test the activation of the GAP promoter and the ADH1 promoter in the construct.