Difference between revisions of "Part:BBa K5143026"
 
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     <h1>Description</h1>
 
     <h1>Description</h1>
 
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         The Alphafactor-venus composite part was used in a larger composite part that enables our team to test the transcriptional activity of GAP promoter and to test the secretion of the protein by the alphafactor.
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         The Alphafactor-venus composite part was used in a larger composite part <a href="https://parts.igem.org/Part:BBa_K5143027" target="_blank">BBa_K5143027</a>that enables our team to test the transcriptional activity of GAP promoter and to test the secretion of the protein by the alphafactor.
 
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            <img src="https://static.igem.wiki/teams/5143/bba-k5143015-venus.png" width="600" alt="Venus fluorescence test">
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            <figcaption><i>Figure 1: Promoter activation test by measuring the Venus fluorescence</i> </figcaption>
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     <h1>Construction</h1>
 
     <h1>Construction</h1>
 
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Alphafactor-Venus is composed of the alphafactor V1 <a href="https://parts.igem.org/Part:BBa_K5143009" target="_blank">BBa_K5143009</a> and the venus chromoprotein <a href="https://parts.igem.org/Part:BBa_K5143015" target="_blank">BBa_K5143015</a>. <br>
 
       Alphafactor-Venus was synthesised and its nucleotide sequence optimised for synthesis and expression in <i>Saccharomyces cerevisiae</i>.  In our construction, Alphafactor-Venus is under the control of gap promoter to test its activation (<a href="https://parts.igem.org/Part:BBa_K4081994" target="_blank">BBa_K4081994</a>).
 
       Alphafactor-Venus was synthesised and its nucleotide sequence optimised for synthesis and expression in <i>Saccharomyces cerevisiae</i>.  In our construction, Alphafactor-Venus is under the control of gap promoter to test its activation (<a href="https://parts.igem.org/Part:BBa_K4081994" target="_blank">BBa_K4081994</a>).
 
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     <h1>References</h1>
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        1) 1. Qin, X. et al. GAP Promoter Library for Fine-Tuning of Gene Expression in Pichia pastoris. Appl Environ Microbiol 77, 3600–3608 (2011). <br>
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2) https://pubmed.ncbi.nlm.nih.gov/25871405 <br>
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3) https://parts.igem.org/Part:BBa_K5143015 <br>
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<h1>Sequence and Features</h1>
 
<h1>Sequence and Features</h1>
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<partinfo>BBa_K5143026SequenceAndFeatures</partinfo>
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<partinfo>BBa_K5143026 SequenceAndFeatures</partinfo>
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<br><h1>References</h1>
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    <p>
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        1) 1. Qin, X. et al. GAP Promoter Library for Fine-Tuning of Gene Expression in Pichia pastoris. Appl Environ Microbiol 77, 3600–3608 (2011). <br>
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2) https://pubmed.ncbi.nlm.nih.gov/25871405 <br>
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3) https://parts.igem.org/Part:BBa_K5143015 <br>
  
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<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  

Latest revision as of 15:37, 1 October 2024


Alphafactor-Venus, a secreted chromoprotein

Protein Description

Description

The Alphafactor-venus composite part was used in a larger composite part BBa_K5143027that enables our team to test the transcriptional activity of GAP promoter and to test the secretion of the protein by the alphafactor.

Venus fluorescence test
Figure 1: Promoter activation test by measuring the Venus fluorescence

Construction

Alphafactor-Venus is composed of the alphafactor V1 BBa_K5143009 and the venus chromoprotein BBa_K5143015.
Alphafactor-Venus was synthesised and its nucleotide sequence optimised for synthesis and expression in Saccharomyces cerevisiae. In our construction, Alphafactor-Venus is under the control of gap promoter to test its activation (BBa_K4081994).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

References

1) 1. Qin, X. et al. GAP Promoter Library for Fine-Tuning of Gene Expression in Pichia pastoris. Appl Environ Microbiol 77, 3600–3608 (2011).
2) https://pubmed.ncbi.nlm.nih.gov/25871405
3) https://parts.igem.org/Part:BBa_K5143015