Difference between revisions of "Part:BBa K5366022:Design"
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===Source=== | ===Source=== | ||
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===References=== | ===References=== | ||
Latest revision as of 15:36, 27 September 2024
T7 promoter-RBS-AJC7-6xHis -T7 termonator
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1534
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 540
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1042
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We ensured that the forward and reverse primer had 20 bp of homology to the plasmid.We needed to purify the AJC7 protein, so we attached the 6×His tag after AJC7.
Source
Pseudothermotoga hypogea DSM