Difference between revisions of "Part:BBa K5143010"
 
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     <img src="https://static.igem.wiki/teams/5143/bba-k5143009-sc-protein-a.png" width="600" alt="α-mating factor" class="center-image">
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     <img src="https://static.igem.wiki/teams/5143/bba-k5143009-and-10-sc-protein.png" width="600" alt="α-mating factor" class="center-image">
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     <h1>Construction</h1>
 
     <h1>Construction</h1>
 
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he Alpha-Factor gene was synthesised and its nucleotide sequence optimised for DNA synthesis and expression in <i>Saccharomyces cerevisiae</i>.
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The Alpha-Factor gene was synthesised and its nucleotide sequence optimised for DNA synthesis and expression in <i>Saccharomyces cerevisiae</i>.
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The gene exists in 2 versions: which limits homologous recombination in the <i>Saccharomyces cerevisiae</i> genome. The second version can be found here : <a href="https://parts.igem.org/Part:BBa_K5143010" target="_blank">BBa_K5143010</a>
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The gene exists in 2 versions: which limits homologous recombination in the <i>Saccharomyces cerevisiae</i> genome. The second version can be found here : <a href="https://parts.igem.org/Part:BBa_K5143009" target="_blank">BBa_K5143009</a>
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<br>These two versions of the alpha-factor can be found in the following composite part:       </p>
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<br>These two versions of the alpha-factor can be found in the following composite part: <a href="https://parts.igem.org/Part:BBa_K5143022" target="_blank">BBa_K5143022</a>       </p>
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    <h1>References</h1>
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Brake, A. J. et al. Alpha-factor-directed synthesis and secretion of mature foreign proteins in Saccharomyces cerevisiae. Proceedings of the National Academy of Sciences 81, 4642–4646 (1984).
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Aza, P., Molpeceres, G., de Salas, F. & Camarero, S. Design of an improved universal signal peptide based on the α-factor mating secretion signal for enzyme production in yeast. Cell Mol Life Sci 78, 3691–3707 (2021).
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<h1>Sequence and Features</h1>
 
<h1>Sequence and Features</h1>
 
<partinfo>BBa_K5143010 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K5143010 SequenceAndFeatures</partinfo>
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<h1>References</h1>
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    <p>
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Brake, A. J. et al. Alpha-factor-directed synthesis and secretion of mature foreign proteins in Saccharomyces cerevisiae. Proceedings of the National Academy of Sciences 81, 4642–4646 (1984).<br>
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<br>
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Aza, P., Molpeceres, G., de Salas, F. & Camarero, S. Design of an improved universal signal peptide based on the α-factor mating secretion signal for enzyme production in yeast. Cell Mol Life Sci 78, 3691–3707 (2021).
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<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  

Latest revision as of 13:57, 1 October 2024

Alpha-Factor secretion signal V2 optimized for expression in Saccharomyces cerevisiae

Protein Description

Description

The α-mating factor is a peptide hormone produced by the yeast Saccharomyces cerevisiae, which is crucial for the mating process of these yeast cells. Here are the key details about the α-mating factor:

  • Function: It is involved in the mating process of S. cerevisiae by being secreted by α-type yeast cells to attract a-type cells, facilitating the mating interaction.
  • Production: Synthesized as a pre-protein that includes a signal peptide and the mature α-mating factor peptide.
  • Structure and Processing:
    • Pre-Pro-α-Mating Factor: Initially synthesized with a signal peptide (pre-region) that directs the nascent protein to the secretory pathway.
    • Mature Peptide: After cleavage of the signal peptide, the remaining peptide is processed into the active α-mating factor.
    • Processing: Includes cleavage of the signal peptide and pro-region to yield the mature peptide.
  • Secretion:
    • Signal Peptide Function: Directs the protein to the endoplasmic reticulum (ER), where it is cleaved for passage through the secretory pathway.
    • Mature Peptide Secretion: Transported through the Golgi apparatus and secreted into the extracellular environment.
  • Applications in Biotechnology:
    • Protein Secretion: The α-mating factor's signal peptide is used to enhance the secretion of various proteins in yeast by attaching it to a protein of interest.
    • Engineering Yeast Strains: Yeast strains can be engineered for efficient production of heterologous proteins by incorporating the α-mating factor signal peptide into their expression constructs.

α-mating factor

Construction

The Alpha-Factor gene was synthesised and its nucleotide sequence optimised for DNA synthesis and expression in Saccharomyces cerevisiae. The gene exists in 2 versions: which limits homologous recombination in the Saccharomyces cerevisiae genome. The second version can be found here : BBa_K5143009
These two versions of the alpha-factor can be found in the following composite part: BBa_K5143022


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 51
    Illegal NheI site found at 211
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

Brake, A. J. et al. Alpha-factor-directed synthesis and secretion of mature foreign proteins in Saccharomyces cerevisiae. Proceedings of the National Academy of Sciences 81, 4642–4646 (1984).

Aza, P., Molpeceres, G., de Salas, F. & Camarero, S. Design of an improved universal signal peptide based on the α-factor mating secretion signal for enzyme production in yeast. Cell Mol Life Sci 78, 3691–3707 (2021).