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= Origin =
 
= Origin =
  
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*E. coli*; synthesized
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E. coli; synthesized
  
 
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The plasmid pSCm-N20 is a genetic construct containing specific DNA sequences that enable the expression of a particular gene or genes in a host organism. It includes a promoter region for gene expression and possibly other regulatory elements necessary for the function of the inserted gene. It is used as the vector plasmid in our research.
 
The plasmid pSCm-N20 is a genetic construct containing specific DNA sequences that enable the expression of a particular gene or genes in a host organism. It includes a promoter region for gene expression and possibly other regulatory elements necessary for the function of the inserted gene. It is used as the vector plasmid in our research.
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= Experimental Approach =
 
  
 
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     <img src="https://static.igem.wiki/teams/5351/bba-k5351000/1.png" width="50%" style="display:block; margin:auto;" alt="Plasmid map of pSCm-N20" >
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     <img src="https://static.igem.wiki/teams/5351/bba-k5351000/1.png" width="30%" style="display:block; margin:auto;" alt="Plasmid map of pSCm-N20" >
 
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         <caption>Figure 1. Plasmid map of pSCm-N20.</caption>
 
         <caption>Figure 1. Plasmid map of pSCm-N20.</caption>
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The plasmid pSCm was digested to obtain a linear backbone with a length of 5984 bp.
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= Experimental Approach =
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The plasmid pSCm was digested to obtain a linear backbone with a length of 5984 bp.The band shown in Figure 2 indicates that the electrophoresis results are consistent with the expected plasmid size, confirming successful linearization of the plasmid backbone.
  
 
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     <img src="https://static.igem.wiki/teams/5351/bba-k5351000/2.jpg" width="50%" style="display:block; margin:auto;" alt="The gel electrophoresis validation of the linear pSCm-N20 plasmid backbone" >
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     <img src="https://static.igem.wiki/teams/5351/bba-k5351000/2.jpg" width="30%" style="display:block; margin:auto;" alt="The gel electrophoresis validation of the linear pSCm-N20 plasmid backbone" >
 
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         <caption>Figure 2. The gel electrophoresis validation of the linear pSCm-N20 plasmid backbone.</caption>
 
         <caption>Figure 2. The gel electrophoresis validation of the linear pSCm-N20 plasmid backbone.</caption>
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The band shown in Figure 2 indicates that the electrophoresis results are consistent with the expected plasmid size, confirming successful linearization of the plasmid backbone.
 

Latest revision as of 06:24, 29 September 2024

pSCm-N20



Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal NheI site found at 1135
    Illegal NheI site found at 4384
    Illegal NotI site found at 2401
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BamHI site found at 2246
    Illegal BamHI site found at 2357
    Illegal XhoI site found at 2408
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.


Origin

E. coli; synthesized

Properties

The plasmid pSCm-N20 is a genetic construct containing specific DNA sequences that enable the expression of a particular gene or genes in a host organism. It includes a promoter region for gene expression and possibly other regulatory elements necessary for the function of the inserted gene. It is used as the vector plasmid in our research.

Plasmid map of pSCm-N20
Figure 1. Plasmid map of pSCm-N20.

Experimental Approach

The plasmid pSCm was digested to obtain a linear backbone with a length of 5984 bp.The band shown in Figure 2 indicates that the electrophoresis results are consistent with the expected plasmid size, confirming successful linearization of the plasmid backbone.

The gel electrophoresis validation of the linear pSCm-N20 plasmid backbone
Figure 2. The gel electrophoresis validation of the linear pSCm-N20 plasmid backbone.