Difference between revisions of "Part:BBa K247004:Experience"

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This part properly acted as RFP reporter.
 
This part properly acted as RFP reporter.
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Excitation peak: 584 nm, Emission peak: 607 nm
  
  
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'''Condition'''
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The Chiba iGEM team tested this part in several conditions.
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'''Experiment Condition'''
  
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AHL concentration : 100 nM
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*AHL concentration of medium : 100 nM
  
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Time :24 h (since we permeated AHL to this RFP)
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*Time when we observed mRFP fluorescence : 24 h
  
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medium : 1 or 5% agar and 10 mL of LB-Amp, Cm medium
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*medium : 10 mL of LB-Amp,Cm medium containing 1(left) or 5(right) %(w/v) agar
  
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[[Image:Chiba_BBa_K247004.png|thumb|right|'''Fig. 1　RFP Fluorescent'''. RFP expression is induced by AHL (100 nM). Observed under UV irradiation. ]]
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[[Image:Chiba_BBa_K247004.png|thumb|left|'''Fig. 1　RFP Fluorescent'''. RFP expression is induced by AHL (100 nM). Observed under UV irradiation.]]

Latest revision as of 04:09, 22 October 2009

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Please enter how you used this part and how it worked out.

Applications of BBa_K247004

User Reviews

UNIQa21574871f5ea34a-partinfo-00000000-QINU UNIQa21574871f5ea34a-partinfo-00000001-QINU

This part properly acted as RFP reporter.

Excitation peak: 584 nm, Emission peak: 607 nm


The Chiba iGEM team tested this part in several conditions.

Experiment Condition

  • AHL concentration of medium : 100 nM
  • Time when we observed mRFP fluorescence : 24 h
  • medium : 10 mL of LB-Amp,Cm medium containing 1(left) or 5(right) %(w/v) agar
Fig. 1　RFP Fluorescent. RFP expression is induced by AHL (100 nM). Observed under UV irradiation.