Difference between revisions of "Part:BBa K5477021"

 
 
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<partinfo>BBa_K5477021 short</partinfo>
 
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CYP3A4-MYC refers to a variant of the CYP3A4 enzyme that has been tagged with a MYC epitope. The MYC tag is a short peptide sequence derived from the c-MYC oncoprotein, and it is widely used to facilitate the detection, purification, or localization of tagged proteins using anti-MYC antibodies.
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CYP3A4-MYC refers to a variant of the CYP3A4 enzyme that has been tagged with a MYC epitope. CYP3A4 (Cytochrome P450 3A4) is one of the enzymes in the cytochrome P450 family responsible for the metabolism of a wide variety of xenobiotics, including drugs, environmental chemicals, and endogenous compounds (2) (4). CYP3A4 metabolizes approximately 50% of all clinically used drugs (1). CYP3A4 is predominantly found in the liver and the intestines, where it plays a role in phase I metabolism (2). CYP3A4 catalyzes the oxygenation of its substrates (2).
  
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CYP3A4, as a part, was integrated into a detoxification module where it initiates the oxidative breakdown of toxic compounds, such as pharmaceuticals and environmental pollutants, allowing these substances to be further processed and excreted (4). This part was combined with POR forming this composite  [https://parts.igem.org/Part:BBa_K5477039  BBa_K5477039].
  
 
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<partinfo>BBa_K5477021 parameters</partinfo>
 
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===References===
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1. Bardal SK, Waechter JE, Martin DS. Chapter 6 - Pharmacogenetics. In: Bardal SK, Waechter JE, Martin DS, editors. Applied Pharmacology [Internet]. Philadelphia: W.B. Saunders; 2011. p. 53–8. Available from: https://www.sciencedirect.com/science/article/pii/B9781437703108000063
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2. Guengerich, F.. (2008). Cytochrome P450 and Chemical Toxicology. Chemical research in toxicology. 21. 70-83. 10.1021/tx700079z.
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3. Klyushova LS, Perepechaeva ML, Grishanova AY. The Role of CYP3A in Health and Disease. Biomedicines. 2022 Oct 24;10(11):2686. doi: 10.3390/biomedicines10112686. PMID: 36359206; PMCID: PMC9687714.
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4. Lynch T, Price A. The effect of cytochrome P450 metabolism on drug response, interactions, and adverse effects. Am Fam Physician. 2007;76(3):391-396.

Latest revision as of 00:34, 2 October 2024


CYP3A4-MYC - Cytochrome P450 3A4 codon-optimized with MYC tag for Saccharomyces cerevisiae

CYP3A4-MYC refers to a variant of the CYP3A4 enzyme that has been tagged with a MYC epitope. CYP3A4 (Cytochrome P450 3A4) is one of the enzymes in the cytochrome P450 family responsible for the metabolism of a wide variety of xenobiotics, including drugs, environmental chemicals, and endogenous compounds (2) (4). CYP3A4 metabolizes approximately 50% of all clinically used drugs (1). CYP3A4 is predominantly found in the liver and the intestines, where it plays a role in phase I metabolism (2). CYP3A4 catalyzes the oxygenation of its substrates (2).

CYP3A4, as a part, was integrated into a detoxification module where it initiates the oxidative breakdown of toxic compounds, such as pharmaceuticals and environmental pollutants, allowing these substances to be further processed and excreted (4). This part was combined with POR forming this composite BBa_K5477039.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1483
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1540
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1483
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1483
  • 1000
    COMPATIBLE WITH RFC[1000]


References

1. Bardal SK, Waechter JE, Martin DS. Chapter 6 - Pharmacogenetics. In: Bardal SK, Waechter JE, Martin DS, editors. Applied Pharmacology [Internet]. Philadelphia: W.B. Saunders; 2011. p. 53–8. Available from: https://www.sciencedirect.com/science/article/pii/B9781437703108000063

2. Guengerich, F.. (2008). Cytochrome P450 and Chemical Toxicology. Chemical research in toxicology. 21. 70-83. 10.1021/tx700079z.

3. Klyushova LS, Perepechaeva ML, Grishanova AY. The Role of CYP3A in Health and Disease. Biomedicines. 2022 Oct 24;10(11):2686. doi: 10.3390/biomedicines10112686. PMID: 36359206; PMCID: PMC9687714.

4. Lynch T, Price A. The effect of cytochrome P450 metabolism on drug response, interactions, and adverse effects. Am Fam Physician. 2007;76(3):391-396.