Difference between revisions of "Part:BBa K5492021"

Latest revision as of 11:37, 2 October 2024

Genscript adapter reverse primer

Reverse primer sequence of the adapter sequence utilised by GenScript. Utilised in PCR reactions for part BBa_K5492402 and BBa_K5492403.

Experiments

PCR – HNMT

We have found the following PCR protocol the most optimal for the part:

According to the previous PCR reaction we decided to use S1 and N1 (from our previous reactions.) We added the following substrates in order: (each optimized and wild type)

We added 6X DNA Loading dye to each PCR tubes. We loaded the wells of the gels with 12μL of DNA solution in the following order:

1. S1O (optimized) 2. S1W (wild type) 3. N1O (optimized) 4. N1W (wild type) 5. Ladder

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 11: / Line 11: @@
 <!-- -->
-<span class='h3bb'>Sequence and Features</span>
+===Experiments===
+==PCR – HNMT==
+We have found the following PCR protocol the most optimal for the part:
+https://static.igem.wiki/teams/5492/registry/hnmt-pcr-plan.png
+According to the previous PCR reaction we decided to use S1 and N1 (from our previous
+reactions.)
+We added the following substrates in order: (each optimized and wild type)
+https://static.igem.wiki/teams/5492/registry/hnmt-pcr-substr1.png
+We added 6X DNA Loading dye to each PCR tubes.
+We loaded the wells of the gels with 12μL of DNA solution in the following order:
+https://static.igem.wiki/teams/5492/registry/hnmt-pcr.png
+. S1O (optimized)
+. S1W (wild type)
+. N1O (optimized)
+. N1W (wild type)
+. Ladder
 <partinfo>BBa_K5492021 SequenceAndFeatures</partinfo>