Difference between revisions of "Part:BBa K5477018"
 
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<partinfo>BBa_K5477018 short</partinfo>
 
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UDPD (UDP-glucose dehydrogenase) is an enzyme that plays a pivotal role in the biosynthesis of UDP-glucuronic acid, which is an essential precursor for the glucuronidation process carried out by UDP-glucuronosyltransferase (UGT) enzymes. Specifically, UDPD catalyzes the oxidation of UDP-glucose to UDP-glucuronic acid, a reaction that supplies the glucuronic acid moiety used by UGT enzymes like UGT2B15 for the detoxification of various substances. Glucuronidation is a critical detoxification mechanism in the body’s phase II metabolism, where lipophilic (fat-soluble) compounds, such as drugs, environmental toxins, and endogenous substances like hormones, are conjugated with glucuronic acid to make them more water-soluble. This transformation facilitates their excretion from the body via urine or bile. UDP-glucuronic acid, produced by UDPD, is a central molecule in this process because it serves as the donor of glucuronic acid for UGT enzymes (1).
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In our detoxification modules, UDPD is an essential enzyme that provides the necessary substrate for the glucuronidation reactions where UGT1A1 and UGT2B15 were used. The following composites and devices, where this part was used, is listed below:  
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UDP-glucose dehydrogenase (UDPD) biosynthesizes UDP-glucuronic acid from UDP-Glucose. UDP-glucuronic acid is a needed substrate in the phase II detoxification reactions carried out by UDP-glucuronosyltransferase (UGT). <i>Saccharomyces cerevisiae</i> has a large pool of UDP-glucose but lacks the ability to synthesize UDP-glucoronic acid (1). This sequence is from <i>Arabidopsis thaliana </i> - At1g26570/T1K7_6.
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Due to the use of UGTs in the detoxification modules, UDPD is co-expressed to provide the necessary substrate for the glucuronidation reactions where UGT1A1 and UGT2B15 were used (1) (2). The following composites and devices, where this part was used, are listed below: [https://parts.igem.org/Part:BBa_K5477035  BBa_K5477035], [https://parts.igem.org/Part:BBa_K5477036  BBa_K5477036], [https://parts.igem.org/Part:BBa_K5477040  BBa_K5477040] and [https://parts.igem.org/Part:BBa_K5477046  BBa_K5477046] and [https://parts.igem.org/Part:BBa_K5477047  BBa_K5477047].
  
  
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===References===
 
===References===
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1. Rowland A, Miners JO, Mackenzie PI. The UDP-glucuronosyltransferases: their role in drug metabolism and detoxification. Int J Biochem Cell Biol. 2013;45(6):1121-1132. doi:10.1016/j.biocel.2013.02.019
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1. Oka T, Jigami Y. Reconstruction of de novo pathway for synthesis of UDP-glucuronic acid and UDP-xylose from intrinsic UDP-glucose in Saccharomyces cerevisiae. FEBS J. 2006 Jun;273(12):2645–57.
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2. Rowland A, Miners JO, Mackenzie PI. The UDP-glucuronosyltransferases: their role in drug metabolism and detoxification. Int J Biochem Cell Biol. 2013;45(6):1121-1132. doi:10.1016/j.biocel.2013.02.019

Latest revision as of 23:42, 1 October 2024


UDPD - UDP-glucose dehydrogenase

UDP-glucose dehydrogenase (UDPD) biosynthesizes UDP-glucuronic acid from UDP-Glucose. UDP-glucuronic acid is a needed substrate in the phase II detoxification reactions carried out by UDP-glucuronosyltransferase (UGT). Saccharomyces cerevisiae has a large pool of UDP-glucose but lacks the ability to synthesize UDP-glucoronic acid (1). This sequence is from Arabidopsis thaliana - At1g26570/T1K7_6.

Due to the use of UGTs in the detoxification modules, UDPD is co-expressed to provide the necessary substrate for the glucuronidation reactions where UGT1A1 and UGT2B15 were used (1) (2). The following composites and devices, where this part was used, are listed below: BBa_K5477035, BBa_K5477036, BBa_K5477040 and BBa_K5477046 and BBa_K5477047.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 542
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 542
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1122
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 542
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 542
  • 1000
    COMPATIBLE WITH RFC[1000]


References

1. Oka T, Jigami Y. Reconstruction of de novo pathway for synthesis of UDP-glucuronic acid and UDP-xylose from intrinsic UDP-glucose in Saccharomyces cerevisiae. FEBS J. 2006 Jun;273(12):2645–57.

2. Rowland A, Miners JO, Mackenzie PI. The UDP-glucuronosyltransferases: their role in drug metabolism and detoxification. Int J Biochem Cell Biol. 2013;45(6):1121-1132. doi:10.1016/j.biocel.2013.02.019