Difference between revisions of "Part:BBa K5492403:Design"

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===Source===
 
===Source===
  
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https://www.ncbi.nlm.nih.gov/nuccore/AH003685, [https://parts.igem.org/Part:BBa_K5492401 BBa_K5492401], [https://parts.igem.org/Part:BBa_K5492100 BBa_K5492100]
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https://www.ncbi.nlm.nih.gov/nuccore/AH003685, [https://parts.igem.org/Part:BBa_K5492401 BBa_K5492401],
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===References===
 
===References===
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https://www.genscript.com/gentitan-gene-fragments.html
 

Latest revision as of 06:57, 2 October 2024


Optimised HNMT - pETDUET-1


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 129
    Illegal EcoRI site found at 235
    Illegal XbaI site found at 47
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 129
    Illegal EcoRI site found at 235
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 129
    Illegal EcoRI site found at 235
    Illegal BglII site found at 1015
    Illegal BamHI site found at 123
    Illegal XhoI site found at 1064
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 129
    Illegal EcoRI site found at 235
    Illegal XbaI site found at 47
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 129
    Illegal EcoRI site found at 235
    Illegal XbaI site found at 47
    Illegal NgoMIV site found at 1034
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Restriction sites adapted to pETDUET-1, with adjustions also added for proper framing of the gene.


Source

https://www.ncbi.nlm.nih.gov/nuccore/AH003685, BBa_K5492401,

References