Difference between revisions of "Part:BBa K5477017"

 
 
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UGT1A1 (UDP-glucuronosyltransferase 1A1) plays a crucial role in the detoxification of environmental pollutants such as dioxins, polycyclic aromatic hydrocarbons (PAHs), and polychlorinated biphenyls (PCBs) through glucuronidation, a process where glucuronic acid is added to lipophilic molecules, increasing their solubility. This reaction converts these toxic compounds into more hydrophilic metabolites, making them easier to excrete via bile or urine. By metabolizing dioxins, PAHs, and PCBs, UGT1A1 helps reduce their toxicity and potential to cause cellular damage, particularly within the liver.
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UGT1A1 (UDP-glucuronosyltransferase 1A1) is an enzyme belonging to the UDP-glucuronosyltransferase (UGT) family and plays a role in the detoxification of environmental pollutants such as dioxins and polychlorinated biphenyls (PCBs) through glucuronidation (2). This is a process where glucuronic acid is added to lipophilic molecules, increasing their solubility (1) (2). This reaction converts these toxic compounds into more hydrophilic metabolites, making them easier to excrete.  
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Incorporating UGT1A1 into the detoxification system together with UDP- glucose dehydrogenase aims to detoxify these compounds. The following composites and devices, where this part was used, are listed below: [https://parts.igem.org/Part:BBa_K5477036  BBa_K5477036] and [https://parts.igem.org/Part:BBa_K5477047  BBa_K5477047].
  
  
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===References===
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1. Dhakal K, Gadupudi GS, Lehmler HJ, Ludewig G, Duffel MW, Robertson LW. Sources and toxicities of phenolic polychlorinated biphenyls (OH-PCBs). Environ Sci Pollut Res. 2018 Jun 1;25(17):16277–90.
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2. Kasai N, Sakaki T, Shinkyo R, Ikushiro S ichi, Iyanagi T, Kamao M, et al. SEQUENTIAL METABOLISM OF 2,3,7-TRICHLORODIBENZO-<em>P</em>-DIOXIN (2,3,7-triCDD) BY CYTOCHROME P450 AND UDP-GLUCURONOSYLTRANSFERASE IN HUMAN LIVER MICROSOMES. Drug Metab Dispos. 2004 Aug 1;32(8):870.

Latest revision as of 23:21, 1 October 2024


UGT1A1 - UDP-glucuronosyltransferase 1A1

UGT1A1 (UDP-glucuronosyltransferase 1A1) is an enzyme belonging to the UDP-glucuronosyltransferase (UGT) family and plays a role in the detoxification of environmental pollutants such as dioxins and polychlorinated biphenyls (PCBs) through glucuronidation (2). This is a process where glucuronic acid is added to lipophilic molecules, increasing their solubility (1) (2). This reaction converts these toxic compounds into more hydrophilic metabolites, making them easier to excrete.

Incorporating UGT1A1 into the detoxification system together with UDP- glucose dehydrogenase aims to detoxify these compounds. The following composites and devices, where this part was used, are listed below: BBa_K5477036 and BBa_K5477047.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 706
    Illegal PstI site found at 148
    Illegal PstI site found at 316
    Illegal PstI site found at 619
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 706
    Illegal PstI site found at 148
    Illegal PstI site found at 316
    Illegal PstI site found at 619
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 706
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 706
    Illegal PstI site found at 148
    Illegal PstI site found at 316
    Illegal PstI site found at 619
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 706
    Illegal PstI site found at 148
    Illegal PstI site found at 316
    Illegal PstI site found at 619
  • 1000
    COMPATIBLE WITH RFC[1000]



References

1. Dhakal K, Gadupudi GS, Lehmler HJ, Ludewig G, Duffel MW, Robertson LW. Sources and toxicities of phenolic polychlorinated biphenyls (OH-PCBs). Environ Sci Pollut Res. 2018 Jun 1;25(17):16277–90.

2. Kasai N, Sakaki T, Shinkyo R, Ikushiro S ichi, Iyanagi T, Kamao M, et al. SEQUENTIAL METABOLISM OF 2,3,7-TRICHLORODIBENZO-P-DIOXIN (2,3,7-triCDD) BY CYTOCHROME P450 AND UDP-GLUCURONOSYLTRANSFERASE IN HUMAN LIVER MICROSOMES. Drug Metab Dispos. 2004 Aug 1;32(8):870.