Difference between revisions of "Part:BBa K5477016"

 
 
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UGT2B15 (UDP-glucuronosyltransferase 2B15) is an enzyme belonging to the UDP-glucuronosyltransferase (UGT) family, which plays a role in the phase II metabolism of xenobiotics and endogenous compounds. UGT2B15 is primarily involved in the process of glucuronidation, a biochemical reaction where glucuronic acid is transferred to lipophilic molecules, making them more water-soluble and easier to excrete from the body. This detoxification process is essential for eliminating harmful substances such as drugs, environmental toxins, and metabolic byproducts.
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UGT2B15 (UDP-glucuronosyltransferase 2B15) is an enzyme belonging to the UDP-glucuronosyltransferase (UGT) family, and plays a role in the phase II metabolism of xenobiotics and endogenous compounds (1). UGT2B15 is primarily involved in the process of glucuronidation, a biochemical reaction where glucuronic acid is transferred to lipophilic molecules, making them more water-soluble and easier to excrete from the body.  
  
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UGT2B15 is responsible for the glucuronidation of a wide range of compounds, including bisphenol A (BPA), steroid hormones (like androgens), and pharmaceuticals (2). By conjugating these substances with glucuronic acid, UGT2B15 facilitates their removal via urine or bile, reducing their biological activity and toxicity (3) (4).
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By incorporating UGT2B15 into the detoxification system [https://parts.igem.org/Part:BBa_K5477046  BBa_K5477046], the goal is to metabolize BPA through glucuronidation. UGT2B15 was integrated in the following composites together with UDPD: [https://parts.igem.org/Part:BBa_K5477035  BBa_K5477035] and [https://parts.igem.org/Part:BBa_K5477040  BBa_K5477040].
  
 
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===References===
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1. Green MD, Oturu EM, Tephly TR. Stable expression of a human liver UDP-glucuronosyltransferase (UGT2B15) with activity toward steroid and xenobiotic substrates. Drug Metab Dispos. 1994 Sep-Oct;22(5):799-805. PMID: 7835232.
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2. Hanioka N, Naito T, Narimatsu S. Human UDP-glucuronosyltransferase isoforms involved in bisphenol A glucuronidation. Chemosphere. 2008 Dec 1;74(1):33–6.
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3. Ramírez, Viviana & Gálvez Ontiveros, Yolanda & Porras, Patricia & Martinez-Gonzalez, Luis & Rivas, Ana & Alvarez-Cubero, María. (2021). METABOLIC pathways, alterations in MIRNAS expression and effects of genetic polymorphisms of bisphenol a analogues: A SYSTEMATIC review. Environmental Research. 197. 111062. 10.1016/j.envres.2021.111062.
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4. Street CM, Zhu Z, Finel M, Court MH. Bisphenol-A glucuronidation in human liver and breast: identification of UDP-glucuronosyltransferases (UGTs) and influence of genetic polymorphisms. Xenobiotica Fate Foreign Compd Biol Syst. 2017 Jan;47(1):1–10.

Latest revision as of 23:11, 1 October 2024


UGT2B15 - UDP-glucuronosyltransferase 2B15

UGT2B15 (UDP-glucuronosyltransferase 2B15) is an enzyme belonging to the UDP-glucuronosyltransferase (UGT) family, and plays a role in the phase II metabolism of xenobiotics and endogenous compounds (1). UGT2B15 is primarily involved in the process of glucuronidation, a biochemical reaction where glucuronic acid is transferred to lipophilic molecules, making them more water-soluble and easier to excrete from the body.

UGT2B15 is responsible for the glucuronidation of a wide range of compounds, including bisphenol A (BPA), steroid hormones (like androgens), and pharmaceuticals (2). By conjugating these substances with glucuronic acid, UGT2B15 facilitates their removal via urine or bile, reducing their biological activity and toxicity (3) (4).

By incorporating UGT2B15 into the detoxification system BBa_K5477046, the goal is to metabolize BPA through glucuronidation. UGT2B15 was integrated in the following composites together with UDPD: BBa_K5477035 and BBa_K5477040.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1342
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1342
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1342
    Illegal BamHI site found at 1162
    Illegal BamHI site found at 1456
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1342
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1342
  • 1000
    COMPATIBLE WITH RFC[1000]


References

1. Green MD, Oturu EM, Tephly TR. Stable expression of a human liver UDP-glucuronosyltransferase (UGT2B15) with activity toward steroid and xenobiotic substrates. Drug Metab Dispos. 1994 Sep-Oct;22(5):799-805. PMID: 7835232.

2. Hanioka N, Naito T, Narimatsu S. Human UDP-glucuronosyltransferase isoforms involved in bisphenol A glucuronidation. Chemosphere. 2008 Dec 1;74(1):33–6.

3. Ramírez, Viviana & Gálvez Ontiveros, Yolanda & Porras, Patricia & Martinez-Gonzalez, Luis & Rivas, Ana & Alvarez-Cubero, María. (2021). METABOLIC pathways, alterations in MIRNAS expression and effects of genetic polymorphisms of bisphenol a analogues: A SYSTEMATIC review. Environmental Research. 197. 111062. 10.1016/j.envres.2021.111062.


4. Street CM, Zhu Z, Finel M, Court MH. Bisphenol-A glucuronidation in human liver and breast: identification of UDP-glucuronosyltransferases (UGTs) and influence of genetic polymorphisms. Xenobiotica Fate Foreign Compd Biol Syst. 2017 Jan;47(1):1–10.