Difference between revisions of "Part:BBa K5246052"

 
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<partinfo>BBa_K5246052 short</partinfo>
 
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===Introduction===
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Vilnius-Lithuania iGEM 2024 project <HTML><b><a href="https://2024.igem.wiki/vilnius-lithuania" target="_blank">Synhesion</a></b></html> aspires to create biodegradable and environmentally friendly adhesives. We were inspired by bacteria, which naturally produce adhesives made from polysaccharides. Two bacteria from aquatic environments - <I> C. crescentus </I> and <I> H. baltica </I> - harness 12 protein synthesis pathways to produce sugars, anchoring them to the surfaces. We aimed to transfer the polysaccharide synthesis pathway to industrially used <I>E. coli</I> bacteria to produce adhesives. Our team concomitantly focused on creating a novel <I>E. coli</I> strain for more efficient production of adhesives.
  
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<HTML>used in Vilnius-Lithuania iGEM 2024 project "Synhesion" <HTML><b><a href="https://2024.igem.wiki/vilnius-lithuania" target="_blank">https://2024.igem.wiki/vilnius-lithuania/</a></b></html>
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===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
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===Sequence and Features===
 
<partinfo>BBa_K5246052 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K5246052 SequenceAndFeatures</partinfo>
  
  
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===Experimental characterization===
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===Functional Parameters===
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<partinfo>BBa_K5246052 parameters</partinfo>
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===References===

Latest revision as of 22:12, 1 October 2024


H.Baltica hfsA-hfsB-hfsD-hfsF-hfsC-hfsI Polysaccharide export apparatus

Introduction

Vilnius-Lithuania iGEM 2024 project Synhesion aspires to create biodegradable and environmentally friendly adhesives. We were inspired by bacteria, which naturally produce adhesives made from polysaccharides. Two bacteria from aquatic environments - C. crescentus and H. baltica - harness 12 protein synthesis pathways to produce sugars, anchoring them to the surfaces. We aimed to transfer the polysaccharide synthesis pathway to industrially used E. coli bacteria to produce adhesives. Our team concomitantly focused on creating a novel E. coli strain for more efficient production of adhesives.

used in Vilnius-Lithuania iGEM 2024 project "Synhesion" https://2024.igem.wiki/vilnius-lithuania/


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 6069
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7303
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2647
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 6069
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 6069
    Illegal NgoMIV site found at 2186
    Illegal AgeI site found at 5527
    Illegal AgeI site found at 6868
  • 1000
    COMPATIBLE WITH RFC[1000]


Experimental characterization

References