Difference between revisions of "Part:BBa K5152008"
 
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<partinfo>BBa_K5152008 short</partinfo>
 
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This device contains the mercury-inducible promoter pMerT and co-expresses its repressor protein MerR, which specifically binds to mercury. When mercury is present, the pMerT promoter drives the expression of the chromoprotein tsPurple, serving as the reporter signal. This device is specifically designed to detect the presence of mercury in the environment.
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This device contains the mercury-inducible promoter pMerT and co-expresses its activator protein MerR, which specifically binds to mercury. When mercury is present, the pMerT promoter drives the expression of the chromoprotein tsPurple, serving as the reporter signal. The design was inspired by iGEM 2010 Team Peking (<partinfo>BBa_K346002</partinfo>).
  
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However, because we ordered the wrong DNA, we didn't have time to clone this construct, and no experiments were conducted on it.
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We used <i>tsPurple</i> as the reporter gene, a purple chromoprotein chosen for its visible colour. This helps create a user-friendly and cost-effective mercury biosensor without needing expensive equipment or special techniques.The illustration of the regulatory mechanism is shown below:
  
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However, because we later found out that we ordered the wrong DNA, we didn't have time to clone this construct, and no experiments were conducted on it.
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===Usage and Biology===
 
===Usage and Biology===
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<span class='h3bb'><b>Sequence and Features</b></span>
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K5152008 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K5152008 SequenceAndFeatures</partinfo>
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Latest revision as of 02:56, 29 September 2024

MerR-pMerT mercury sensing chromoprotein reporter device

This device contains the mercury-inducible promoter pMerT and co-expresses its activator protein MerR, which specifically binds to mercury. When mercury is present, the pMerT promoter drives the expression of the chromoprotein tsPurple, serving as the reporter signal. The design was inspired by iGEM 2010 Team Peking (BBa_K346002).

We used tsPurple as the reporter gene, a purple chromoprotein chosen for its visible colour. This helps create a user-friendly and cost-effective mercury biosensor without needing expensive equipment or special techniques.The illustration of the regulatory mechanism is shown below:

However, because we later found out that we ordered the wrong DNA, we didn't have time to clone this construct, and no experiments were conducted on it.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 35
    Illegal NheI site found at 58
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]