Difference between revisions of "Part:BBa K5152005"
| (2 intermediate revisions by the same user not shown) | |||
| Line 2: | Line 2: | ||
<partinfo>BBa_K5152005 short</partinfo> | <partinfo>BBa_K5152005 short</partinfo> | ||
| − | This part was used to create a cadmium-sensing biosensor for our project. The design of this part was inspired by iGEM 2009 Team Newcastle (<partinfo>BBa_K174015</partinfo>) | + | This part was used to create a cadmium-sensing biosensor for our project. The design of this part was inspired by iGEM 2009 Team Newcastle (<partinfo>BBa_K174015</partinfo>). |
| − | Our goal is to develop heavy metal biosensors that don't require special techniques or expensive equipment, so we used chromoproteins for their visible | + | Our goal is to develop heavy metal biosensors that don't require special techniques or expensive equipment, so we used chromoproteins for their visible colours. |
We designed this part and ordered it from IDT gBlock. However, we consistently failed to obtain successful transformants for unknown reasons. Despite using the same assembly process as other constructs, which succeeded, this one did not. The cause remains unclear. | We designed this part and ordered it from IDT gBlock. However, we consistently failed to obtain successful transformants for unknown reasons. Despite using the same assembly process as other constructs, which succeeded, this one did not. The cause remains unclear. | ||
| + | <!-- | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
| + | <!-- --> | ||
<span class='h3bb'><b>Sequence and Features</b></span> | <span class='h3bb'><b>Sequence and Features</b></span> | ||
Latest revision as of 00:11, 27 September 2024
pCadA cadmium sensing chromoprotein reporter device
This part was used to create a cadmium-sensing biosensor for our project. The design of this part was inspired by iGEM 2009 Team Newcastle (BBa_K174015).
Our goal is to develop heavy metal biosensors that don't require special techniques or expensive equipment, so we used chromoproteins for their visible colours.
We designed this part and ordered it from IDT gBlock. However, we consistently failed to obtain successful transformants for unknown reasons. Despite using the same assembly process as other constructs, which succeeded, this one did not. The cause remains unclear.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 228
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]