Difference between revisions of "Part:BBa K5477005"
 
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<partinfo>BBa_K5477005 short</partinfo>
 
<partinfo>BBa_K5477005 short</partinfo>
  
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The GAL1 and GAL10 promoters are two tightly linked, yet functionally distinct, promoters found in <i>Saccharomyces cerevisiae</i>. They regulate the expression of genes involved in galactose metabolism. The GAL1 promoter drives the expression of the GAL1 gene, which encodes galactokinase, an enzyme responsible for the phosphorylation of galactose. The GAL10 promoter controls the GAL10 gene, encoding UDP-glucose-4-epimerase, another key enzyme in the galactose metabolic pathway (1). Both promoters are induced in the presence of galactose and repressed by glucose through the action of regulatory proteins such as Gal4p and Gal80p. The upstream activating sequences (UAS) located between the two promoters are critical for the activation of both promoters, and Gal4p binds these UAS elements to initiate transcription when galactose is present.  
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The GAL1 and GAL10 promoters are two promoters found in <i>Saccharomyces cerevisiae</i>. They regulate the expression of genes involved in galactose metabolism. The GAL1 promoter drives the expression of the GAL1 gene, which encodes galactokinase, an enzyme responsible for the phosphorylation of galactose. The GAL10 promoter controls the GAL10 gene, encoding UDP-glucose-4-epimerase, another key enzyme in the galactose metabolic pathway (1). Both promoters are induced in the presence of galactose and repressed by glucose through the action of regulatory proteins such as Gal4p and Gal80p. The upstream activating sequences (UAS) located between the two promoters are critical for the activation of both promoters, and Gal4p binds these UAS elements to initiate transcription when galactose is present (2).  
  
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The GAL1/10 bidirectional promoter was formed by combining these two promoters, with the UAS elements located centrally, allowing for the simultaneous regulation of both genes in opposite directions.  
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The GAL1/10 bidirectional promoter was formed by combining these two promoters, with the UAS elements located centrally, allowing for the simultaneous regulation of both genes in opposite directions (1). pGAL1/10 was used to drive the expression of the  detoxification devices. The following composite parts were built:
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<table border="1" cellpadding="10" cellspacing="0">
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    <tr>
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        <th>Constructs</th>
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        <th>Composite Part Name</th>
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    </tr>
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    <tr>
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        <td><i>UDPD-pGAL1/10-UGT2B15</i></td>
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        <td>[https://parts.igem.org/Part:BBa_K5477035  BBa_K5477035]</td>
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    </tr>
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    <tr>
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        <td><i>UDPD-pGAL1/10-UGT1A1</i></td>
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        <td>[https://parts.igem.org/Part:BBa_K5477036  BBa_K5477036]</td>
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    </tr>
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    <tr>
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        <td><i>CYP1A1-pGAL1/10-POR</i></td>
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        <td>[https://parts.igem.org/Part:BBa_K5477037  BBa_K5477037]</td>
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    </tr>
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    <tr>
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        <td><i>CYP3A4-pGAL1/10-POR</i></td>
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        <td>[https://parts.igem.org/Part:BBa_K5477038  BBa_K5477038]</td>
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    </tr>
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    <tr>
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        <td><i>CYP3A4-MYC-pGAL1/10-POR</i></td>
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        <td>[https://parts.igem.org/Part:BBa_K5477039  BBa_K5477039]</td>
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    </tr>
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</table>
  
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pGAL1/10 was used to drive the expression of our detoxification system. The following composite parts were built:
 
  
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UDPD-pGAL1/10-UGT2B15
 
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UDPD-pGAL1/10-UGT1A1
 
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CYP1A1-pGAL1/10-POR
 
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CYP3A4-pGAL1/10-POR
 
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CYP3A4-MYC-pGAL1/10-POR
 
  
  
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===References===
 
===References===
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1. West RW Jr, Chen SM, Putz H, Butler G, Banerjee M. GAL1-GAL10 divergent promoter region of Saccharomyces cerevisiae contains negative control elements in addition to functionally separate and possibly overlapping upstream activating sequences. Genes Dev. 1987 Dec;1(10):1118-31. doi: 10.1101/gad.1.10.1118. PMID: 3322938.
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1. Elison GL, Xue Y, Song R, Acar M. Insights into Bidirectional Gene Expression Control Using the Canonical GAL1/GAL10 Promoter. Cell Rep. 2018 Oct 16;25(3):737-748.e4. doi: 10.1016/j.celrep.2018.09.050. PMID: 30332652; PMCID: PMC6263159.
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2. West RW Jr, Chen SM, Putz H, Butler G, Banerjee M. GAL1-GAL10 divergent promoter region of Saccharomyces cerevisiae contains negative control elements in addition to functionally separate and possibly overlapping upstream activating sequences. Genes Dev. 1987 Dec;1(10):1118-31. doi: 10.1101/gad.1.10.1118. PMID: 3322938.

Latest revision as of 20:24, 1 October 2024


pGAL1/10 - bidirectional inducible promoters from Saccharomyces cerevisiae

The GAL1 and GAL10 promoters are two promoters found in Saccharomyces cerevisiae. They regulate the expression of genes involved in galactose metabolism. The GAL1 promoter drives the expression of the GAL1 gene, which encodes galactokinase, an enzyme responsible for the phosphorylation of galactose. The GAL10 promoter controls the GAL10 gene, encoding UDP-glucose-4-epimerase, another key enzyme in the galactose metabolic pathway (1). Both promoters are induced in the presence of galactose and repressed by glucose through the action of regulatory proteins such as Gal4p and Gal80p. The upstream activating sequences (UAS) located between the two promoters are critical for the activation of both promoters, and Gal4p binds these UAS elements to initiate transcription when galactose is present (2).

The GAL1/10 bidirectional promoter was formed by combining these two promoters, with the UAS elements located centrally, allowing for the simultaneous regulation of both genes in opposite directions (1). pGAL1/10 was used to drive the expression of the detoxification devices. The following composite parts were built:


Constructs Composite Part Name
UDPD-pGAL1/10-UGT2B15 BBa_K5477035
UDPD-pGAL1/10-UGT1A1 BBa_K5477036
CYP1A1-pGAL1/10-POR BBa_K5477037
CYP3A4-pGAL1/10-POR BBa_K5477038
CYP3A4-MYC-pGAL1/10-POR BBa_K5477039



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 374
  • 1000
    COMPATIBLE WITH RFC[1000]


References

1. Elison GL, Xue Y, Song R, Acar M. Insights into Bidirectional Gene Expression Control Using the Canonical GAL1/GAL10 Promoter. Cell Rep. 2018 Oct 16;25(3):737-748.e4. doi: 10.1016/j.celrep.2018.09.050. PMID: 30332652; PMCID: PMC6263159.

2. West RW Jr, Chen SM, Putz H, Butler G, Banerjee M. GAL1-GAL10 divergent promoter region of Saccharomyces cerevisiae contains negative control elements in addition to functionally separate and possibly overlapping upstream activating sequences. Genes Dev. 1987 Dec;1(10):1118-31. doi: 10.1101/gad.1.10.1118. PMID: 3322938.