Difference between revisions of "Part:BBa K5382124"

Latest revision as of 15:29, 28 September 2024

CL7-A protein tag

CL7 is a protein tag engineered from colicin CE7. In E.coli, CE7 is a bacteriocin that belongs to the colicin DNase family and has endonuclease activity. Through engineering, CL7 removed DNA binding and catalytic activity, but retained its high affinity binding ability to the corresponding inhibitory protein Im7. This property makes CL7 a useful tool for protein purification and molecular biology applications.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 133
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 133
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 133
Illegal BglII site found at 81
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 133
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 133
Illegal AgeI site found at 31
Illegal AgeI site found at 45
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K5382124 short</partinfo>
-CL7 is an engineered variant of colicin CE7, which is a protein tag. In Escherichia coli, CE7 is a bacteriocin that belongs to the colicin DNase family and has endonuclease activity. Through engineering, CL7 removed DNA binding and catalytic activity, but retained its high affinity binding ability to the corresponding inhibitory protein Im7. This property makes CL7 a useful tool for protein purification and molecular biology applications.
+CL7 is a protein tag engineered from colicin CE7. In E.coli, CE7 is a bacteriocin that belongs to the colicin DNase family and has endonuclease activity. Through engineering, CL7 removed DNA binding and catalytic activity, but retained its high affinity binding ability to the corresponding inhibitory protein Im7. This property makes CL7 a useful tool for protein purification and molecular biology applications.
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