Difference between revisions of "Part:BBa K5291027"

Latest revision as of 00:55, 2 October 2024

nadK: nicotinamide adenine dinucleotide kinase

nadK encodes NAD kinases(NADK). NADK can use ATP as their phosphoryl donor to phosphorylate NAD+. We used it to increase the intracellular levels of NADP+.

Usage and Biology

In carbon fixation module we constructed pBBR1MCS2-nadM-nadK-pntA-pntB to improve the intracellular NADPH and NADP⁺ level.

Fig.1 The map of plasmid pBBR1MCS2-nadM-nadK-pntA-pntB.

We successfully cloned the nadM-nadK gene by PCR.

Fig.2 Electropherogram of nadM-nadK PCR gel.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K5291027 short</partinfo>
-nadK encodes NAD kinases(NADK). NADK can use ATP as their phosphoryl donor to phosphorylate NAD+. We used it to increase the intracellular levels of NADP+.
+<i>nadK</i> encodes NAD kinases(NADK). NADK can use ATP as their phosphoryl donor to phosphorylate NAD+. We used it to increase the intracellular levels of NADP+.
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
+In carbon fixation module we constructed pBBR1MCS2-<i>nadM-nadK-pntA-pntB</i> to improve the intracellular NADPH and NADP<sup>+</sup> level.<br>
+<html><img width = "600" src="https://static.igem.wiki/teams/5291/images/parts-lhs/pbbr1mcs2-pnta-pntb-nadm-nadk.png" /></html><br>
+<b>Fig.1 The map of plasmid pBBR1MCS2-<i>nadM-nadK-pntA-pntB</i>.</b><br><br>
+We successfully cloned the <i>nadM-nadK</i> gene by PCR.<br>
+<html><img width = "300" src="https://static.igem.wiki/teams/5291/images/result/electropherogram-of-nadm-nadk-pcr-gel.png" /></html><html><img width = "500" src="https://static.igem.wiki/teams/5291/images/parts-lhs/nadm-nadk-pnta-pntb.png" /></html><br>
+<b>Fig.2 Electropherogram of <i>nadM-nadK</i> PCR gel.</b>
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