Difference between revisions of "Part:BBa K243011:Design"

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===Design Notes===
 
===Design Notes===
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The cloning steps were planed theoretically before we started the work in the wet lab.
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The cloning steps were planned theoretically before we started the work in the wet lab.
−
 
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We used the [https://parts.igem.org/Part:BBa_K243006 long linker] because its lenght of 36bp is a bit shorter than the split linker we used.
 +
We had to find a compromise between connection stability and possible sterical interference between the single parts.
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The parts are fused according to [https://parts.igem.org/Assembly_standard_25 RFC 25].
 
[https://static.igem.org/mediawiki/parts/2/24/Freiburg09_Strediglonglifoki.txt Commented GenBank file]
 
[https://static.igem.org/mediawiki/parts/2/24/Freiburg09_Strediglonglifoki.txt Commented GenBank file]
  

Latest revision as of 21:00, 21 October 2009

Strep-DigA-Long Linker-Fok_i


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 278
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The cloning steps were planned theoretically before we started the work in the wet lab. We used the long linker because its lenght of 36bp is a bit shorter than the split linker we used. We had to find a compromise between connection stability and possible sterical interference between the single parts. The parts are fused according to RFC 25. Commented GenBank file

Source

Combined the parts by serial cloning steps.

References