Difference between revisions of "Part:BBa K5047000:Design"
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===Source=== | ===Source=== | ||
| − | + | This part was synthetically designed. The main sequence was taken from a piece of DNA from Crispr interference. This part comes from Schearli Lab. | |
===References=== | ===References=== | ||
Latest revision as of 18:33, 24 September 2024
shRNAv1 : DNA sequence encodes a shRNA that does not bind to any genes. It serves as a control.
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 63
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The sequence was chosen to lack any significant homology to functional genes in the model organism, thereby avoiding off-target effects making it an appropriate negative control.
Source
This part was synthetically designed. The main sequence was taken from a piece of DNA from Crispr interference. This part comes from Schearli Lab.