Difference between revisions of "Part:BBa K5115001"
| Line 11: | Line 11: | ||
It's used to build up the hydrogenase which reduces the nickel ion. In our experiment, it was constructed into our ribozyme-assisted polycistronic co-expression system [https://parts.igem.org/Part:BBa_K5115011 BBa_K5115011(ribozyme+RBS+hoxF+stem-loop)]. And then it will eventually make up parts [https://parts.igem.org/Part:BBa_K5115066 BBa_K5115066(mineral, U module)]and [https://parts.igem.org/Part:BBa_K5115067 BBa_K5115067(mineral, F module)], which are used for further experiment. | It's used to build up the hydrogenase which reduces the nickel ion. In our experiment, it was constructed into our ribozyme-assisted polycistronic co-expression system [https://parts.igem.org/Part:BBa_K5115011 BBa_K5115011(ribozyme+RBS+hoxF+stem-loop)]. And then it will eventually make up parts [https://parts.igem.org/Part:BBa_K5115066 BBa_K5115066(mineral, U module)]and [https://parts.igem.org/Part:BBa_K5115067 BBa_K5115067(mineral, F module)], which are used for further experiment. | ||
| + | |||
| + | ===Sequence and Features=== | ||
<!-- --> | <!-- --> | ||
<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
Latest revision as of 06:19, 2 October 2024
hoxF, without stop codon
Introduction
The hoxF is a subunit of the hox and hyp operon BBa_K5115020.
Usage and Biology
It's used to build up the hydrogenase which reduces the nickel ion. In our experiment, it was constructed into our ribozyme-assisted polycistronic co-expression system BBa_K5115011(ribozyme+RBS+hoxF+stem-loop). And then it will eventually make up parts BBa_K5115066(mineral, U module)and BBa_K5115067(mineral, F module), which are used for further experiment.
Sequence and Features
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 577
Illegal BglII site found at 1395
Illegal BglII site found at 1688
Illegal XhoI site found at 22 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 456
Illegal NgoMIV site found at 994
Illegal NgoMIV site found at 1204
Illegal NgoMIV site found at 1516 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 625
Illegal BsaI.rc site found at 1129