Difference between revisions of "Part:BBa K5115010"
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<partinfo>BBa_K5115010 short</partinfo> | <partinfo>BBa_K5115010 short</partinfo> | ||
| − | <html><img style="float:right;width:128px" src="https://static.igem.wiki/teams/5115/czh/mineral-logo.svg" alt="contributed by Fudan iGEM | + | <html><img style="float:right;width:128px" src="https://static.igem.wiki/teams/5115/czh/mineral-logo.svg" alt="contributed by Fudan iGEM 2024"></html> |
__TOC__ | __TOC__ | ||
===Introduction=== | ===Introduction=== | ||
| − | The hypF is a subunit of the hox and hyp operon[https://parts.igem.org/Part:BBa_K5115020 BBa_K5115020]. | + | The hypF is a subunit of the hox and hyp operon [https://parts.igem.org/Part:BBa_K5115020 BBa_K5115020]. |
===Usage and Biology=== | ===Usage and Biology=== | ||
It's used to build up the hydrogenase which reduces the nickel ion. In our experiment, it was constructed into our ribozyme-assisted polycistronic co-expression system [https://parts.igem.org/Part:BBa_K5115019 BBa_K5115019(ribozyme+RBS+hypF+stem-loop)]. And then it will eventually make up parts [https://parts.igem.org/Part:BBa_K5115066 BBa_K5115066(mineral, U module)]and [https://parts.igem.org/Part:BBa_K5115067 BBa_K5115067(mineral, F module)], which are used for further experiments. | It's used to build up the hydrogenase which reduces the nickel ion. In our experiment, it was constructed into our ribozyme-assisted polycistronic co-expression system [https://parts.igem.org/Part:BBa_K5115019 BBa_K5115019(ribozyme+RBS+hypF+stem-loop)]. And then it will eventually make up parts [https://parts.igem.org/Part:BBa_K5115066 BBa_K5115066(mineral, U module)]and [https://parts.igem.org/Part:BBa_K5115067 BBa_K5115067(mineral, F module)], which are used for further experiments. | ||
| − | + | ===Sequence and Features=== | |
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
Latest revision as of 13:13, 2 October 2024
hypF
Introduction
The hypF is a subunit of the hox and hyp operon BBa_K5115020.
Usage and Biology
It's used to build up the hydrogenase which reduces the nickel ion. In our experiment, it was constructed into our ribozyme-assisted polycistronic co-expression system BBa_K5115019(ribozyme+RBS+hypF+stem-loop). And then it will eventually make up parts BBa_K5115066(mineral, U module)and BBa_K5115067(mineral, F module), which are used for further experiments.
Sequence and Features
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 424
Illegal XhoI site found at 2263 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 29
Illegal NgoMIV site found at 250
Illegal NgoMIV site found at 274
Illegal NgoMIV site found at 635
Illegal NgoMIV site found at 2035
Illegal NgoMIV site found at 2435 - 1000COMPATIBLE WITH RFC[1000]