Difference between revisions of "Part:BBa K5115026"

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<partinfo>BBa_K5115026 short</partinfo>
 
<partinfo>BBa_K5115026 short</partinfo>
  
<html><img style="float:right;width:128px" src="https://static.igem.wiki/teams/5115/czh/mineral-logo.svg" alt="contributed by Fudan iGEM 2023"></html>
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<html><img style="float:right;width:128px" src="https://static.igem.wiki/teams/5115/czh/mineral-logo.svg" alt="contributed by Fudan iGEM 2024"></html>
 
__TOC__
 
__TOC__
 
===Introduction===
 
===Introduction===
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The csoS2 is a subunit of the csoS operon [https://parts.igem.org/Part:BBa_K5115034 BBa_K5115034].
  
 
===Usage and Biology===
 
===Usage and Biology===
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The csoS2 is a critical protein involved in the assembly of the α-carboxysome, specifically in organizing the shell and encapsulating cargo enzymes. In our system, when combined with these other components, csoS2 ensures the correct formation of the empty carboxysome structure and plays a crucial role in incorporating target enzymes inside the shell. Together with the other csoS subunits, csoS2 helps create a functional, enclosed environment for biotechnological applications, such as enzyme encapsulation for improved catalytic efficiency.
  
 
===Characterization===
 
===Characterization===
  
 
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===Sequence and Features===
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
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==References==
 
==References==
<references />
 
[1] Li, T., Jiang, Q., Huang, J., Aitchison, C. M., Huang, F., Yang, M., Dykes, G. F., He, H. L., Wang, Q., Sprick, R. S., Cooper, A. I., & Liu, L. N. (2020). Reprogramming bacterial protein organelles as a nanoreactor for hydrogen production. Nature communications, 11(1), 5448. https://doi.org/10.1038/s41467-020-19280-0
 
 
[2] Oltrogge, L. M., Chaijarasphong, T., Chen, A. W., Bolin, E. R., Marqusee, S., & Savage, D. F. (2020). Multivalent interactions between CsoS2 and Rubisco mediate α-carboxysome formation. Nature structural & molecular biology, 27(3), 281–287. https://doi.org/10.1038/s41594-020-0387-7
 

Latest revision as of 13:18, 2 October 2024


csoS2

contributed by Fudan iGEM 2024

Introduction

The csoS2 is a subunit of the csoS operon BBa_K5115034.

Usage and Biology

The csoS2 is a critical protein involved in the assembly of the α-carboxysome, specifically in organizing the shell and encapsulating cargo enzymes. In our system, when combined with these other components, csoS2 ensures the correct formation of the empty carboxysome structure and plays a crucial role in incorporating target enzymes inside the shell. Together with the other csoS subunits, csoS2 helps create a functional, enclosed environment for biotechnological applications, such as enzyme encapsulation for improved catalytic efficiency.

Characterization

Sequence and Features

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 133
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 291
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 799
    Illegal AgeI site found at 1750
    Illegal AgeI site found at 2431
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 191


References