Difference between revisions of "Part:BBa K5115003"
 
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<partinfo>BBa_K5115003 short</partinfo>
 
<partinfo>BBa_K5115003 short</partinfo>
  
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<html><img style="float:right;width:128px" src="https://static.igem.wiki/teams/5115/czh/mineral-logo.svg" alt="contributed by Fudan iGEM 2023"></html>
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<html><img style="float:right;width:128px" src="https://static.igem.wiki/teams/5115/czh/mineral-logo.svg" alt="contributed by Fudan iGEM 2024"></html>
 
__TOC__
 
__TOC__
 
===Introduction===
 
===Introduction===
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The hoxU is a subunit of the hox and hyp operon[https://parts.igem.org/Part:BBa_K5115020 BBa_K5115020].
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The hoxU is a subunit of the hox and hyp operon [https://parts.igem.org/Part:BBa_K5115020 BBa_K5115020].
  
 
===Usage and Biology===
 
===Usage and Biology===
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It's used to buid up the hydrogenase which ruduces the nickel ion. In our experiment, it was put into the ribozyme system[https://parts.igem.org/Part:BBa_K5115012 BBa_K5115012]in case that it can be expressed smoothly in a polucostronic sequences.
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It's used to build up the hydrogenase which reduces the nickel ion. In our experiment, it was constructed into our ribozyme-assisted polycistronic co-expression system [https://parts.igem.org/Part:BBa_K5115012 BBa_K5115012(ribozyme+RBS+hoxU+stem-loop)]. And then it will eventually make up parts [https://parts.igem.org/Part:BBa_K5115066 BBa_K5115066(mineral, U module)]and [https://parts.igem.org/Part:BBa_K5115067 BBa_K5115067(mineral, F module)], which are used for further experiment.
  
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===Sequence and Features===
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Latest revision as of 06:49, 2 October 2024


hoxU, without stop codon

contributed by Fudan iGEM 2024

Introduction

The hoxU is a subunit of the hox and hyp operon BBa_K5115020.

Usage and Biology

It's used to build up the hydrogenase which reduces the nickel ion. In our experiment, it was constructed into our ribozyme-assisted polycistronic co-expression system BBa_K5115012(ribozyme+RBS+hoxU+stem-loop). And then it will eventually make up parts BBa_K5115066(mineral, U module)and BBa_K5115067(mineral, F module), which are used for further experiment.

Sequence and Features

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 410
    Illegal BglII site found at 488
    Illegal XhoI site found at 418
    Illegal XhoI site found at 610
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 371
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 192
    Illegal BsaI site found at 354
    Illegal SapI.rc site found at 303


References