Difference between revisions of "Part:BBa K216017:Design"
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− | * Xi, L., Cho, K.W., and Tu, S.C. 1991. Cloning and nucleotide sequences of ''lux | + | * Xi, L., Cho, K.W., and Tu, S.C. 1991. Cloning and nucleotide sequences of ''lux'' genes and characterization of luciferase of ''Xenorhabdus luminescens'' from a human wound. ''Journal of Bacteriology'' '''173''', 1399-1405. |
* Meighen, E.A., and Szittner, R.B. 1992. Multiple repetitive elements and organization of the ''lux'' operons of luminescent terrestrial bacteria. '' J. Bacteriol.'' '''174''', 5371-5381. | * Meighen, E.A., and Szittner, R.B. 1992. Multiple repetitive elements and organization of the ''lux'' operons of luminescent terrestrial bacteria. '' J. Bacteriol.'' '''174''', 5371-5381. |
Latest revision as of 16:49, 21 October 2009
luxCDE genes of Xenorhabdus luminescens (aldehyde biosynthesis)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 2410
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 3042
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1332
Design Notes
The normal operon structure is luxCDABE. This part is derived from an earlier (non-BioBrick) project, in which the luxCD genes were fused to luxE so that the aldehyde biosynthesis machinery could be induced separately from the luciferase. An XbaI site must be removed from within luxD.
Source
As part of an earlier project, the luxCD and luxE regions were separately amplified from genomic DNA of Xenorhabdus luminescens Hb (NCIMB 12670) and joined at a NotI site to create an artificially reduced luxCDE operon. This was tested and was found to work well in E. coli. The part shown here was made by amplifying this using primers which added the BioBrick prefix and suffix.
References
- Xi, L., Cho, K.W., and Tu, S.C. 1991. Cloning and nucleotide sequences of lux genes and characterization of luciferase of Xenorhabdus luminescens from a human wound. Journal of Bacteriology 173, 1399-1405.
- Meighen, E.A., and Szittner, R.B. 1992. Multiple repetitive elements and organization of the lux operons of luminescent terrestrial bacteria. J. Bacteriol. 174, 5371-5381.