Difference between revisions of "Part:BBa K5115003"

Latest revision as of 06:49, 2 October 2024

hoxU, without stop codon

It's used to build up the hydrogenase which reduces the nickel ion. In our experiment, it was constructed into our ribozyme-assisted polycistronic co-expression system BBa_K5115012(ribozyme+RBS+hoxU+stem-loop). And then it will eventually make up parts BBa_K5115066(mineral, U module)and BBa_K5115067(mineral, F module), which are used for further experiment.

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 410
Illegal BglII site found at 488
Illegal XhoI site found at 418
Illegal XhoI site found at 610
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 371
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 192
Illegal BsaI site found at 354
Illegal SapI.rc site found at 303

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K5115003 short</partinfo>
-<html><img style="float:right;width:128px" src="https://static.igem.wiki/teams/5115/czh/mineral-logo.svg" alt="contributed by Fudan iGEM 2023"></html>
+<html><img style="float:right;width:128px" src="https://static.igem.wiki/teams/5115/czh/mineral-logo.svg" alt="contributed by Fudan iGEM 2024"></html>
 __TOC__
 ===Introduction===
@@ Line 9: / Line 9: @@
 ===Usage and Biology===
-It's used to buid up the hydrogenase which ruduces the nickel ion.
+It's used to build up the hydrogenase which reduces the nickel ion. In our experiment, it was constructed into our ribozyme-assisted polycistronic co-expression system [https://parts.igem.org/Part:BBa_K5115012 BBa_K5115012(ribozyme+RBS+hoxU+stem-loop)]. And then it will eventually make up parts [https://parts.igem.org/Part:BBa_K5115066 BBa_K5115066(mineral, U module)]and [https://parts.igem.org/Part:BBa_K5115067 BBa_K5115067(mineral, F module)], which are used for further experiment.
+===Sequence and Features===
 <!-- -->
 <span class='h3bb'>Sequence and Features</span>