Difference between revisions of "Part:BBa K5374023:Design"

 
 
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__NOTOC__
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<partinfo>BBa_K5374023 short</partinfo>
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<partinfo>BBa_K5374023 SequenceAndFeatures</partinfo>
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===Design Notes===
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The sequence was optimized for EGFP fusion, ensuring the domain’s collagen-binding function while maintaining fluorescence for activity evaluation.
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===Source===
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VWF-A domain from von Willebrand factor, with EGFP from BBa_K1911005.

Latest revision as of 11:57, 24 September 2024


VWF-A Domain-EGFP (von Willebrand Factor A Domain). A domain known for mediating collagen binding, p


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 112
    Illegal PstI site found at 875
    Illegal PstI site found at 1282
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1330
    Illegal PstI site found at 112
    Illegal PstI site found at 875
    Illegal PstI site found at 1282
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 849
    Illegal BamHI site found at 1017
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 112
    Illegal PstI site found at 875
    Illegal PstI site found at 1282
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 112
    Illegal PstI site found at 875
    Illegal PstI site found at 1282
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1973


Design Notes

The sequence was optimized for EGFP fusion, ensuring the domain’s collagen-binding function while maintaining fluorescence for activity evaluation.


Source

VWF-A domain from von Willebrand factor, with EGFP from BBa_K1911005.