Difference between revisions of "Part:BBa K5374022:Design"
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+ | __NOTOC__ | ||
+ | <partinfo>BBa_K5374022 short</partinfo> | ||
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+ | <partinfo>BBa_K5374022 SequenceAndFeatures</partinfo> | ||
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+ | ===Design Notes=== | ||
+ | Sequence was optimized to maintain COMP’s collagen-binding properties while preserving EGFP’s fluorescence for effective functional analysis. | ||
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+ | |||
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+ | ===Source=== | ||
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+ | Derived from COMP gene, with EGFP from BBa_K1911005. |
Latest revision as of 11:52, 24 September 2024
COMP-EGFP (Cartilage Oligomeric Matrix Protein). A protein that binds and stabilizes collagen struct
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 133
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1306
Illegal PstI site found at 133 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 133
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 133
Illegal AgeI site found at 355 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1949
Design Notes
Sequence was optimized to maintain COMP’s collagen-binding properties while preserving EGFP’s fluorescence for effective functional analysis.
Source
Derived from COMP gene, with EGFP from BBa_K1911005.