Difference between revisions of "Part:BBa K5115027"

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<partinfo>BBa_K5115027 short</partinfo>
 
<partinfo>BBa_K5115027 short</partinfo>
  
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<html><img style="float:right;width:128px" src="https://static.igem.wiki/teams/5115/czh/mineral-logo.svg" alt="contributed by Fudan iGEM 2023"></html>
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<html><img style="float:right;width:128px" src="https://static.igem.wiki/teams/5115/czh/mineral-logo.svg" alt="contributed by Fudan iGEM 2024"></html>
 
__TOC__
 
__TOC__
 
===Introduction===
 
===Introduction===
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CsoSCA is a critical component of the carboxysome in cyanobacteria, specifically identified in Cyanobium sp. PCC7001. It plays a vital role in carbon concentrating mechanisms by enhancing CO2 fixation through its catalytic activity as a carbonic anhydrase (CA). Recent studies reveal that CsoSCA is allosterically activated by ribulose-1,5-bisphosphate (RuBP), underscoring its regulatory significance in photosynthesis.
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The csoS3 is a subunit of the original csoS operon.
  
 
===Usage and Biology===
 
===Usage and Biology===
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CsoSCA's functionality is tightly linked to the presence of RuBP, which is essential for its enzymatic activity. It forms a hexameric structure that allows efficient CO2 hydration, enhancing the overall effectiveness of the carboxysome. Understanding the regulation and structure of CsoSCA is crucial for advancing knowledge in cyanobacterial biology and improving carbon fixation strategies in biotechnology applications.
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The csoS3 encodes the β-carbonic anhydrase enzyme, which is not necessary in our design, therefore removed from the csoS operon [https://parts.igem.org/Part:BBa_K5115034 BBa_K5115034], while other subunits will still be used to generate a synthetic α-carboxysome shell in ''E. coli''.
  
 
===Characterization===
 
===Characterization===
  
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===Sequence and Features===
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
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==References==
 
==References==
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<references />
 

Latest revision as of 13:21, 2 October 2024


csoS3

contributed by Fudan iGEM 2024

Introduction

The csoS3 is a subunit of the original csoS operon.

Usage and Biology

The csoS3 encodes the β-carbonic anhydrase enzyme, which is not necessary in our design, therefore removed from the csoS operon BBa_K5115034, while other subunits will still be used to generate a synthetic α-carboxysome shell in E. coli.

Characterization

Sequence and Features

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 146
  • 1000
    COMPATIBLE WITH RFC[1000]


References