Difference between revisions of "Part:BBa K5186003:Design"

Latest revision as of 08:18, 24 September 2024

sRNA3(IspH)

Assembly Compatibility:

Synthetic oligo

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−	~~===Design Notes===~~
−	In E. coli, IspH is an essential enzyme in the MEP pathway, crucial to isoprenoid synthesis which helps maintain, stabilize and support core functions such as respiration. It catalyzes the conversion of HMBPP into the common isoprenoid precursors IPP and DMAPP in a single step. And it has been confirmed that significantly inhibiting the IspH activity in E. coli substantially resctricts the bacterial growth (Kumar et al.). To achieve a balance between HMBPP overproduction and growth activity of E. coli, we decided on the sRNA-based down-regulation of IspH instead of the knockout approach.