Difference between revisions of "Part:BBa K5115026"

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<partinfo>BBa_K5115026 short</partinfo>
 
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<html><img style="float:right;width:128px" src="https://static.igem.wiki/teams/5115/czh/mineral-logo.svg" alt="contributed by Fudan iGEM 2023"></html>
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<html><img style="float:right;width:128px" src="https://static.igem.wiki/teams/5115/czh/mineral-logo.svg" alt="contributed by Fudan iGEM 2024"></html>
 
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===Introduction===
 
===Introduction===
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CsoS2 is an essential protein involved in the assembly and formation of α-carboxysomes, which are bacterial microcompartments designed to encapsulate enzymes such as ribulose-1,5-bisphosphate carboxylase-oxygenase (Rubisco) for carbon fixation. The carboxysome shell, composed of various proteins, serves as a selectively permeable barrier, facilitating the flux of specific metabolites while protecting enzymes from external inhibitors like oxygen. CsoS2 plays a crucial role in mediating the self-assembly of the shell and is functionally analogous to linking proteins found in β-carboxysomes, like CcmM. In the chemoautotrophic bacterium ''Halothiobacillus neapolitanus'', CsoS2 is present in two isoforms (CsoS2A and CsoS2B), which together drive the formation of ordered, virus-like protein shells in ''E. coli''.
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The csoS2 is a subunit of the csoS operon [https://parts.igem.org/Part:BBa_K5115034 BBa_K5115034].
  
 
===Usage and Biology===
 
===Usage and Biology===
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In the assembly of α-carboxysomes, CsoS2 is vital not only for shell formation but also for cargo encapsulation. This protein interacts with other shell components to organize the structure and recruit internal enzymes. The N-terminal region of CsoS2 facilitates interactions with Rubisco, ensuring its appropriate encapsulation within the carboxysome. CsoS2's C-terminal region acts as an encapsulation peptide (EP), linking the shell to external enzymes and directing their incorporation into the microcompartment. This mechanism was demonstrated by fusing CsoS2 with enhanced green fluorescent protein (GFP) in ''E. coli'', which resulted in the formation of highly organized shell structures capable of encapsulating GFP-tagged proteins.
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The csoS2 is a critical protein involved in the assembly of the α-carboxysome, specifically in organizing the shell and encapsulating cargo enzymes. In our system, when combined with these other components, csoS2 ensures the correct formation of the empty carboxysome structure and plays a crucial role in incorporating target enzymes inside the shell. Together with the other csoS subunits, csoS2 helps create a functional, enclosed environment for biotechnological applications, such as enzyme encapsulation for improved catalytic efficiency.
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The functional importance of CsoS2 has also been highlighted in studies on hydrogenase encapsulation. By leveraging the shell's ability to create an oxygen-limited environment, CsoS2-mediated shells were used to encapsulate oxygen-sensitive [FeFe]-hydrogenases, demonstrating enhanced catalytic performance for hydrogen production. This encapsulation improved the enzymes' oxygen tolerance and preserved their catalytic activity, making CsoS2 a valuable tool in the construction of synthetic nanoreactors for biohydrogen production and other biotechnological applications.
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===Characterization===
 
===Characterization===
  
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===Sequence and Features===
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
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==References==
 
==References==
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<references />
 

Latest revision as of 13:18, 2 October 2024


csoS2

contributed by Fudan iGEM 2024

Introduction

The csoS2 is a subunit of the csoS operon BBa_K5115034.

Usage and Biology

The csoS2 is a critical protein involved in the assembly of the α-carboxysome, specifically in organizing the shell and encapsulating cargo enzymes. In our system, when combined with these other components, csoS2 ensures the correct formation of the empty carboxysome structure and plays a crucial role in incorporating target enzymes inside the shell. Together with the other csoS subunits, csoS2 helps create a functional, enclosed environment for biotechnological applications, such as enzyme encapsulation for improved catalytic efficiency.

Characterization

Sequence and Features

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 133
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 291
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 799
    Illegal AgeI site found at 1750
    Illegal AgeI site found at 2431
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 191


References