Difference between revisions of "Part:BBa K5115026"

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<partinfo>BBa_K5115026 short</partinfo>
 
<partinfo>BBa_K5115026 short</partinfo>
  
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<html><img style="float:right;width:128px" src="https://static.igem.wiki/teams/5115/czh/mineral-logo.svg" alt="contributed by Fudan iGEM 2023"></html>
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<html><img style="float:right;width:128px" src="https://static.igem.wiki/teams/5115/czh/mineral-logo.svg" alt="contributed by Fudan iGEM 2024"></html>
 
__TOC__
 
__TOC__
 
===Introduction===
 
===Introduction===
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CsoS2 is an essential protein involved in the assembly and formation of α-carboxysomes, which are bacterial microcompartments designed to encapsulate enzymes such as ribulose-1,5-bisphosphate carboxylase-oxygenase (Rubisco) for carbon fixation. The carboxysome shell, composed of various proteins, serves as a selectively permeable barrier, facilitating the flux of specific metabolites while protecting enzymes from external inhibitors like oxygen. CsoS2 plays a crucial role in mediating the self-assembly of the shell and is functionally analogous to linking proteins found in β-carboxysomes, like CcmM. In the chemoautotrophic bacterium ''Halothiobacillus neapolitanus'', CsoS2 is present in two isoforms (CsoS2A and CsoS2B), which together drive the formation of ordered, virus-like protein shells in ''E. coli''.
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The csoS2 is a subunit of the csoS operon [https://parts.igem.org/Part:BBa_K5115034 BBa_K5115034].
  
 
===Usage and Biology===
 
===Usage and Biology===
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The csoS2 is a critical protein involved in the assembly of the α-carboxysome, specifically in organizing the shell and encapsulating cargo enzymes. In our system, when combined with these other components, csoS2 ensures the correct formation of the empty carboxysome structure and plays a crucial role in incorporating target enzymes inside the shell. Together with the other csoS subunits, csoS2 helps create a functional, enclosed environment for biotechnological applications, such as enzyme encapsulation for improved catalytic efficiency.
  
 
===Characterization===
 
===Characterization===
  
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===Sequence and Features===
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
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==References==
 
==References==
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<references />
 

Latest revision as of 13:18, 2 October 2024


csoS2

contributed by Fudan iGEM 2024

Introduction

The csoS2 is a subunit of the csoS operon BBa_K5115034.

Usage and Biology

The csoS2 is a critical protein involved in the assembly of the α-carboxysome, specifically in organizing the shell and encapsulating cargo enzymes. In our system, when combined with these other components, csoS2 ensures the correct formation of the empty carboxysome structure and plays a crucial role in incorporating target enzymes inside the shell. Together with the other csoS subunits, csoS2 helps create a functional, enclosed environment for biotechnological applications, such as enzyme encapsulation for improved catalytic efficiency.

Characterization

Sequence and Features

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 133
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 291
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 799
    Illegal AgeI site found at 1750
    Illegal AgeI site found at 2431
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 191


References