Difference between revisions of "Part:BBa K5071022"

 
 
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pRSFDuet-1 backbone
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    <h2>BBa_K5071022 (pRSFDuet-1 backbone)</h2>
  
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    <h3>Name</h3>
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===Functional Parameters===
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    <p>pRSFDuet-1 backbone</p>
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<partinfo>BBa_K5071022 parameters</partinfo>
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    <h3>Base Pairs</h3>
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    <p>3587 bp</p>
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    <h3>Origin</h3>
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    <p>Escherichia coli</p>
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    <h3>Usage and Biology</h3>
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    <p>
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        The pRSFDuet-1 backbone is a plasmid vector obtained by linearizing the plasmid pRSFD, and we used this vector for the connection of the target genes.
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    </p>
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    <h3>Cultivation</h3>
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        The pRSFDuet-1 backbone is a plasmid vector obtained by linearizing the plasmid pRSFD, with a length of 3587 bp. Fig 1. shows a band consistent with the target size, indicating that the target gene was successfully amplified. After agarose gel electrophoresis and gel recovery, homologous recombination was used to obtain the recombinant plasmid.
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        <img src="https://static.igem.wiki/teams/5071/bba-k5071022/1.jpg" alt="Fig 1. The purpose segment of backbone pRSFDuet-1">
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        <div class="caption">Fig 1. The purpose segment of backbone pRSFDuet-1</div>
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Latest revision as of 06:12, 30 September 2024


pRSFDuet-1 backbone


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal NotI site found at 149
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BglII site found at 305
    Illegal BamHI site found at 106
    Illegal XhoI site found at 354
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal NgoMIV site found at 324
    Illegal AgeI site found at 566
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.

<!DOCTYPE html> BBa_K5071022 (pRSFDuet-1 backbone)

BBa_K5071022 (pRSFDuet-1 backbone)

Name

pRSFDuet-1 backbone

Base Pairs

3587 bp

Origin

Escherichia coli

Usage and Biology

The pRSFDuet-1 backbone is a plasmid vector obtained by linearizing the plasmid pRSFD, and we used this vector for the connection of the target genes.

Cultivation

The pRSFDuet-1 backbone is a plasmid vector obtained by linearizing the plasmid pRSFD, with a length of 3587 bp. Fig 1. shows a band consistent with the target size, indicating that the target gene was successfully amplified. After agarose gel electrophoresis and gel recovery, homologous recombination was used to obtain the recombinant plasmid.

Fig 1. The purpose segment of backbone pRSFDuet-1
Fig 1. The purpose segment of backbone pRSFDuet-1