Difference between revisions of "Part:BBa K5071021"

 
 
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<partinfo>BBa_K5071021 short</partinfo>
 
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pETDuet-1 backbone
 
  
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===Usage and Biology===
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===Functional Parameters===
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    <h2>BBa_K5071021 (pETDuet-1 backbone)</h2>
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    <h3>Name</h3>
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    <p>pETDuet-1 backbone</p>
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    <h3>Base Pairs</h3>
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    <p>5178 bp</p>
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    <h3>Origin</h3>
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    <p>Escherichia coli</p>
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    <h3>Usage and Biology</h3>
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    <p>
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        The pETDuet-1 backbone is a plasmid vector obtained by linearizing the plasmid pETD, and we used this vector for the connection of the target genes.
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    </p>
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    <h3>Cultivation</h3>
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    <p>
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        The pETDuet-1 backbone is a plasmid vector obtained by linearizing the plasmid pETD, with a length of 5178 bp. Fig 1. shows a band consistent with the target size, indicating that the target gene was successfully amplified. After agarose gel electrophoresis and gel recovery, homologous recombination was used to obtain the recombinant plasmid.
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        <img src="https://static.igem.wiki/teams/5071/bba-k5071021/1.jpg" alt="Fig 1. The purpose segment of backbone pETDuet-1">
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        <div class="caption">Fig 1. The purpose segment of backbone pETDuet-1</div>
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Latest revision as of 06:24, 30 September 2024

pETDuet-1 backbone



Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal NotI site found at 149
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BglII site found at 305
    Illegal BamHI site found at 106
    Illegal XhoI site found at 354
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal NgoMIV site found at 324
    Illegal NgoMIV site found at 671
    Illegal NgoMIV site found at 5348
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.


BBa_K5071021 (pETDuet-1 backbone)

BBa_K5071021 (pETDuet-1 backbone)

Name

pETDuet-1 backbone

Base Pairs

5178 bp

Origin

Escherichia coli

Usage and Biology

The pETDuet-1 backbone is a plasmid vector obtained by linearizing the plasmid pETD, and we used this vector for the connection of the target genes.

Cultivation

The pETDuet-1 backbone is a plasmid vector obtained by linearizing the plasmid pETD, with a length of 5178 bp. Fig 1. shows a band consistent with the target size, indicating that the target gene was successfully amplified. After agarose gel electrophoresis and gel recovery, homologous recombination was used to obtain the recombinant plasmid.

Fig 1. The purpose segment of backbone pETDuet-1
Fig 1. The purpose segment of backbone pETDuet-1