Difference between revisions of "Part:BBa K5299200:Design"
Mkakadiari (Talk | contribs) |
Mkakadiari (Talk | contribs) (→Design Notes) |
||
| (3 intermediate revisions by the same user not shown) | |||
| Line 6: | Line 6: | ||
===Design Notes=== | ===Design Notes=== | ||
| − | + | Created using the Golden Braid method, that utilises type IIS enzymes. We used BsmBI to create Level 0 constructs, and BsaI for Level 1 constructs. | |
| − | + | ||
===Source=== | ===Source=== | ||
Promoter is BBa_K4583008, originally from [1].<br /> | Promoter is BBa_K4583008, originally from [1].<br /> | ||
| − | RBS is BBa_B0030. <br /> | + | RBS is BBa_B0030. <br /> |
sfGFP is BBa_I746916, originally from [2]. <br /> | sfGFP is BBa_I746916, originally from [2]. <br /> | ||
| − | Terminator is BBa_B0015. | + | Terminator is BBa_B0015. |
| − | + | ||
| − | + | ||
===References=== | ===References=== | ||
| + | *[1]: Jaishankar, J., & Srivastava, P. (2020). Strong synthetic stationary phase promoter-based gene expression system for Escherichia coli. <i> Plasmid, 109 </i>(102491), 102491. doi:10.1016/j.plasmid.2020.102491 | ||
| + | *[2]: Pédelacq, J.-D., Cabantous, S., Tran, T., Terwilliger, T. C., & Waldo, G. S. (2006). Engineering and characterization of a superfolder green fluorescent protein. <i> Nature Biotechnology, 24 </i>(1), 79–88. doi:10.1038/nbt1172 | ||
Latest revision as of 07:47, 27 September 2024
P3.1 - BBa_B0030 - BBa_I746916 - BBa_B0015
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 259
Design Notes
Created using the Golden Braid method, that utilises type IIS enzymes. We used BsmBI to create Level 0 constructs, and BsaI for Level 1 constructs.
Source
Promoter is BBa_K4583008, originally from [1].
RBS is BBa_B0030.
sfGFP is BBa_I746916, originally from [2].
Terminator is BBa_B0015.
References
- [1]: Jaishankar, J., & Srivastava, P. (2020). Strong synthetic stationary phase promoter-based gene expression system for Escherichia coli. Plasmid, 109 (102491), 102491. doi:10.1016/j.plasmid.2020.102491
- [2]: Pédelacq, J.-D., Cabantous, S., Tran, T., Terwilliger, T. C., & Waldo, G. S. (2006). Engineering and characterization of a superfolder green fluorescent protein. Nature Biotechnology, 24 (1), 79–88. doi:10.1038/nbt1172