Difference between revisions of "Part:BBa K5299200:Design"
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+ | __NOTOC__ | ||
+ | <partinfo>BBa_K5299200 short</partinfo> | ||
+ | <partinfo>BBa_K5299200 SequenceAndFeatures</partinfo> | ||
+ | |||
+ | |||
+ | ===Design Notes=== | ||
+ | Created using the Golden Braid method, that utilises type IIS enzymes. We used BsmBI to create Level 0 constructs, and BsaI for Level α constructs. | ||
+ | |||
+ | ===Source=== | ||
+ | Promoter is BBa_K4583008, originally from [1].<br /> | ||
+ | RBS is BBa_B0030. <br /> | ||
+ | sfGFP is BBa_I746916. <br /> | ||
+ | Terminator is BBa_B0015. | ||
+ | |||
+ | ===References=== | ||
+ | |||
+ | *[1]: Jaishankar, J., & Srivastava, P. (2020). Strong synthetic stationary phase promoter-based gene expression system for Escherichia coli. <i> Plasmid, 109 </i>(102491), 102491. doi:10.1016/j.plasmid.2020.102491 |
Latest revision as of 15:24, 30 September 2024
P3.1 - BBa_B0030 - BBa_I746916 - BBa_B0015
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 259
Design Notes
Created using the Golden Braid method, that utilises type IIS enzymes. We used BsmBI to create Level 0 constructs, and BsaI for Level α constructs.
Source
Promoter is BBa_K4583008, originally from [1].
RBS is BBa_B0030.
sfGFP is BBa_I746916.
Terminator is BBa_B0015.
References
- [1]: Jaishankar, J., & Srivastava, P. (2020). Strong synthetic stationary phase promoter-based gene expression system for Escherichia coli. Plasmid, 109 (102491), 102491. doi:10.1016/j.plasmid.2020.102491