Difference between revisions of "Part:BBa K5208001"
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YtnP encodes for an N-acyl homoserine lactonase in B. subtilis WB600. This enzyme catalyzes the hydrolysis of N-Acyl homoserine lactone (AHL) (Schneider et al., 2012). | YtnP encodes for an N-acyl homoserine lactonase in B. subtilis WB600. This enzyme catalyzes the hydrolysis of N-Acyl homoserine lactone (AHL) (Schneider et al., 2012). | ||
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===Sequence and Features=== | ===Sequence and Features=== | ||
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===Functional Parameters=== | ===Functional Parameters=== | ||
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Latest revision as of 04:52, 23 September 2024
YtnP, N-acyl homoserine lactonase from Bacillus subtilis WB600
YtnP encodes for an N-acyl homoserine lactonase in B. subtilis WB600. This enzyme catalyzes the hydrolysis of N-Acyl homoserine lactone (AHL) (Schneider et al., 2012).
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 558
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Usage and Biology
YtnP degrades AHLs, a class of signaling molecules that induce quorum sensing. Quorum sensing triggers the expression of virulence factors in pathogens, including Aeromonas hydrophila, an aquatic bacterium that threatens aquaculture (Coquant et al., 2020; Hlordzi et al., 2020). High expression of YtnP in the probiotic B. subtilis reduces the virulence of A. hydrophila through quorum quenching and saves aquatic animals.
References
Chen, F., Gao, Y., Chen, X., Yu, Z., & Li, X. (2013). Quorum quenching enzymes and their application in degrading signal molecules to block quorum sensing-dependent infection. Int J Mol Sci, 14(9), 17477-17500. https://doi.org/10.3390/ijms140917477
Coquant, G., Grill, J. P., & Seksik, P. (2020). Impact of N-Acyl-Homoserine Lactones, Quorum Sensing Molecules, on Gut Immunity. Front Immunol, 11, 1827. https://doi.org/10.3389/fimmu.2020.01827
Dong, Y. H., Xu, J. L., Li, X. Z., & Zhang, L. H. (2000). AiiA, an enzyme that inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence of Erwinia carotovora. Proc Natl Acad Sci U S A, 97(7), 3526-3531. https://doi.org/10.1073/pnas.97.7.3526
Hlordzi, V., Kuebutornye, F. K. A., Afriyie, G., Abarike, E. D., Lu, Y., Chi, S., & Anokyewaa, M. A. (2020). The use of Bacillus species in maintenance of water quality in aquaculture: A review. Aquaculture Reports, 18, 100503. https://doi.org/https://doi.org/10.1016/j.aqrep.2020.100503
Schneider, J., Yepes, A., Garcia-Betancur, J. C., Westedt, I., Mielich, B., & López, D. (2012). Streptomycin-induced expression in Bacillus subtilis of YtnP, a lactonase-homologous protein that inhibits development and streptomycin production in Streptomyces griseus. Appl Environ Microbiol, 78(2), 599-603. https://doi.org/10.1128/aem.06992-11