Difference between revisions of "Part:BBa K5324000:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | This sequence was codon optimized for expression in E.coli. Was designed due to current part <partinfo>BBa_K1456006</partinfo>having an excess base pair causing a frame shift along with encoding for more than just aprotinin. | + | This sequence was codon optimized for expression in E.coli. Was designed due to current part <partinfo>BBa_K1456006</partinfo>having an excess base pair causing a frame shift along with encoding for more than just aprotinin. This was made to have the simplified protein without additional modification beyond aprotinin. |
===Source=== | ===Source=== | ||
| − | Genomic sequence obtained from literature. | + | Genomic sequence obtained from literature. Originated from Bos taurus. |
===References=== | ===References=== | ||
| + | 1 - Vedvick, T.S. (1993) ‘DNA for expression of aprotinin in methylotrophic yeast cells’. <br> | ||
| + | 2 - Moitra, V. et al. (2009) ‘What is the best means of preventing perioperative renal injury?’, Evidence-Based Practice of Anesthesiology, pp. 226–239. doi:10.1016/b978-1-4160-5996-7.00034-1. | ||
Latest revision as of 22:09, 29 September 2024
Aprotinin (bovine pancreatic trypsin inhibitor)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This sequence was codon optimized for expression in E.coli. Was designed due to current part BBa_K1456006having an excess base pair causing a frame shift along with encoding for more than just aprotinin. This was made to have the simplified protein without additional modification beyond aprotinin.
Source
Genomic sequence obtained from literature. Originated from Bos taurus.
References
1 - Vedvick, T.S. (1993) ‘DNA for expression of aprotinin in methylotrophic yeast cells’.
2 - Moitra, V. et al. (2009) ‘What is the best means of preventing perioperative renal injury?’, Evidence-Based Practice of Anesthesiology, pp. 226–239. doi:10.1016/b978-1-4160-5996-7.00034-1.