Difference between revisions of "Part:BBa K5286001:Design"
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===Design Notes=== | ===Design Notes=== | ||
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This sequence is the original sequence obtained from the Boothby et al. paper, which was obtained via mRNA retrotrasncription and it has already been codon optimized for expression in E. coli. | This sequence is the original sequence obtained from the Boothby et al. paper, which was obtained via mRNA retrotrasncription and it has already been codon optimized for expression in E. coli. | ||
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===Source=== | ===Source=== | ||
Latest revision as of 21:13, 22 September 2024
Cytosolic-abundant heat soluble protein 94063 (CAHS94063)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 459
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This sequence is the original sequence obtained from the Boothby et al. paper, which was obtained via mRNA retrotrasncription and it has already been codon optimized for expression in E. coli.
Source
The part was obtained via RNAseq of Hypsibius dujardini under desiccation conditions by Boothby et al.(2017)
References
Boothby, T. C., Tapia, H., Brozena, A. H., Piszkiewicz, S., Smith, A. E., Giovannini, I., Rebecchi, L., Pielak, G. J., Koshland, D., & Goldstein, B. (2017). Tardigrades Use Intrinsically Disordered Proteins to Survive Desiccation. Molecular Cell, 65(6), 975. https://doi.org/10.1016/j.molcel.2017.02.018