Difference between revisions of "Part:BBa K201001"
 
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<partinfo>BBa_K201001 short</partinfo>
 
<partinfo>BBa_K201001 short</partinfo>
  
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GFP reporter protein cloned downstream of the BBa_K201008 promoter (constitutive BBa_J23118 promoter plus O2 Lac operator).  
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GFP reporter protein cloned downstream of the BBa_K201008 promoter (constitutive BBa_J23100 promoter plus O2 Lac operator). See the experience section for '''characterization'''.
  
 
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===Usage and Biology===
 
===Usage and Biology===
  
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See the experience section for '''characterization'''.
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
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===Functional Parameters===
 
===Functional Parameters===
 
<partinfo>BBa_K201001 parameters</partinfo>
 
<partinfo>BBa_K201001 parameters</partinfo>
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operator binding affinity for the repressor LacI: K= 1.7 nM
 
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Latest revision as of 01:26, 22 October 2009

LacI repressed GFP generator with BBa_K201008

GFP reporter protein cloned downstream of the BBa_K201008 promoter (constitutive BBa_J23100 promoter plus O2 Lac operator). See the experience section for characterization.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 734