Difference between revisions of "Part:BBa K5107000:Design"

 
 
(2 intermediate revisions by the same user not shown)
Line 6: Line 6:
  
  
−
===Design Notes===
+
===Source===
−
As the system should be transcriptionally functional when the hormone is not present in the solution, we add the di-nucleotides GA between the T7 promoter and the HRE in order to keep high transcription level(extra Gs in the 3' end of the promoter).
+
  
 +
The part was synthesized as a component of the entire device we used in our cell-free system obtaining as G-block from IDT.
  
  
−
===Source===
 
−
 
−
The part is synthesised as g-block part of the the whole device we used in our cell free system by IDT.
 
  
−
===References===
+
‌

Latest revision as of 08:46, 1 October 2024


HRE_minimal


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Source

The part was synthesized as a component of the entire device we used in our cell-free system obtaining as G-block from IDT.


‌