Difference between revisions of "Part:BBa K243002:Design"

(→‎Design Notes)
 
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===Design Notes===
 
===Design Notes===
−
The signal sequence had to be located at the N-terminus of the (fusion)protein.
+
The signal sequence has to be located at the N-terminus of the fused protein.
 
Designed according to [https://parts.igem.org/Assembly_standard_25 RFC 25].<br>
 
Designed according to [https://parts.igem.org/Assembly_standard_25 RFC 25].<br>
 
[https://static.igem.org/mediawiki/parts/d/db/Freiburg09_Dsba.txt Commented GenBank file]
 
[https://static.igem.org/mediawiki/parts/d/db/Freiburg09_Dsba.txt Commented GenBank file]
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===Source===
 
===Source===
  
−
Sequence of the DsbA was copied out from E.coli B genom
+
The amino acid sequence of the DsbA signal sequence was extracted from the ''E. coli'' B genome.
−
Synthesized oligos with signal sequence of DsbA by sigma.  
+
Synthesized oligos encoding the signal sequence of DsbA were designed and eventually ordered from Sigma.  
  
 
===References===
 
===References===
  
 
Steiner, D; Forrer, P; Stumpp, MT,(2006).Signal sequences directing cotranslational translocation expand the range of proteins amenable to phage display ''Nature Biotechnology'' Vol.24 Issue:7 Pages: 823-831
 
Steiner, D; Forrer, P; Stumpp, MT,(2006).Signal sequences directing cotranslational translocation expand the range of proteins amenable to phage display ''Nature Biotechnology'' Vol.24 Issue:7 Pages: 823-831

Latest revision as of 02:58, 22 October 2009

DsbA signal sequence (enables periplasm export)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The signal sequence has to be located at the N-terminus of the fused protein. Designed according to RFC 25.
Commented GenBank file

Source

The amino acid sequence of the DsbA signal sequence was extracted from the E. coli B genome. Synthesized oligos encoding the signal sequence of DsbA were designed and eventually ordered from Sigma.

References

Steiner, D; Forrer, P; Stumpp, MT,(2006).Signal sequences directing cotranslational translocation expand the range of proteins amenable to phage display Nature Biotechnology Vol.24 Issue:7 Pages: 823-831