Difference between revisions of "Part:BBa K283038:Design"
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<partinfo>BBa_K283038 short</partinfo> | <partinfo>BBa_K283038 short</partinfo> | ||
| − | The prosseA promoter with a strong rbs. The prosseA promoter comes from pathogenic bacteria Samonella 7207 strain. It is the Salmonella pathogenicity island II regulator. SsrB is autoregulated and activates expression of SPI-2 and SPI-2 co-regulated genes in response to an unknown signal(s).SsrB is autoregulated and activates expression of SPI-2 and SPI-2 co-regulated genes in response to an unknown signal(s). The preliminary data in our lab shows that the activation this regulator depends on the presence of SsrB protein without SsrA on the chromosome of E.coli and salmonella (single copy). However, when we use this prosseA based on the plasmid (not single copy) it can trigger high gene expression level. | + | The prosseA promoter with a strong rbs. The prosseA promoter comes from pathogenic bacteria ''Samonella'' 7207 strain. It is the ''Salmonella'' pathogenicity island II regulator. SsrB is autoregulated and activates expression of SPI-2 and SPI-2 co-regulated genes in response to an unknown signal(s).SsrB is autoregulated and activates expression of SPI-2 and SPI-2 co-regulated genes in response to an unknown signal(s). The preliminary data in our lab shows that the activation this regulator depends on the presence of SsrB protein without SsrA on the chromosome of ''E.coli'' and ''salmonella'' (single copy). However, when we use this prosseA based on the plasmid (not single copy) it can trigger high gene expression level. |
<partinfo>BBa_K283038 SequenceAndFeatures</partinfo> | <partinfo>BBa_K283038 SequenceAndFeatures</partinfo> | ||
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===Source=== | ===Source=== | ||
| − | This promoter comes from the pathogenic strain Samonella 7207 | + | This promoter comes from the pathogenic strain ''Samonella'' 7207 |
===References=== | ===References=== | ||
Latest revision as of 18:11, 21 October 2009
prosseA promoter
The prosseA promoter with a strong rbs. The prosseA promoter comes from pathogenic bacteria Samonella 7207 strain. It is the Salmonella pathogenicity island II regulator. SsrB is autoregulated and activates expression of SPI-2 and SPI-2 co-regulated genes in response to an unknown signal(s).SsrB is autoregulated and activates expression of SPI-2 and SPI-2 co-regulated genes in response to an unknown signal(s). The preliminary data in our lab shows that the activation this regulator depends on the presence of SsrB protein without SsrA on the chromosome of E.coli and salmonella (single copy). However, when we use this prosseA based on the plasmid (not single copy) it can trigger high gene expression level.
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 372
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 114
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
n/a
Source
This promoter comes from the pathogenic strain Samonella 7207